Methylated Gene Primer Panel for Accurate Colorectal Cancer Detection
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Solution Overview
Problem
Existing methods for diagnosing colorectal cancer lack sensitivity and specificity in detecting methylation of colorectal cancer marker genes, limiting their effectiveness in early detection and diagnosis.
Innovation Solution
Utilizing primers that specifically amplify multiple methylated colorectal cancer marker genes, such as SDC2 and ADHFE1, in combination with reagents that modify methylated and non-methylated DNA, followed by PCR and probe hybridization to enhance detection accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If existing detection methods are used for colorectal cancer diagnosis, then the detection process is simple, but the sensitivity and specificity are insufficient
Solution Approach 1:
The detection process is segmented into distinct functional steps: DNA extraction, methylation-specific PCR amplification using primers designed to target methylated CpG islands, and detection of amplified products. This segmentation allows each step to be optimized independently, improving overall detection accuracy while maintaining procedural clarity.
Solution Approach 2:
The method changes key parameters including using methylation-specific primers with specific sequences that bind only to methylated DNA regions, adjusting PCR conditions to favor amplification of methylated templates, and detecting methylation status rather than gene expression. These parameter changes enable high sensitivity and specificity detection.
2Reliability
If multiple methylated marker genes are detected simultaneously, then the sensitivity and specificity improve, but the detection complexity increases
Solution Approach 1:
Multiple methylation detection assays for different marker genes (such as SDC2, ADHFE1, and other colorectal cancer-associated genes) are merged into a single integrated detection system. The method combines multiple primer sets targeting different CpG islands in one workflow, allowing simultaneous detection of multiple markers without requiring separate detection systems for each gene, thus improving reliability while controlling complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Improves the sensitivity and specificity of colorectal cancer diagnosis by detecting methylation patterns in samples like stool, blood, or plasma, enabling early and accurate detection of the disease.
Implementation Method 1
treating a sample with at least one reagent differently modifying methylated SDC2 (syndecan 2) and ADHFE1 (alcohol dehydrogenase iron-containing 1) genes and non-methylated SDC2 and ADHFE1 genes
Implementation Method 2
performing treatment with primers specifically amplifying the methylated SDC2 and ADHFE1 genes
Implementation Method 3
followed by PCR and probe hybridization to enhance detection accuracy
Data Source
AI summary
The present invention relates to a method for providing information for colorectal cancer diagnosis, a composition for diagnosing colorectal cancer, and a kit comprising same, and, more particularly, to: a method for providing information for colorectal cancer diagnosis by using a primer for specifically amplifying plural methylated colorectal cancer marker genes; a composition for diagnosing colorectal cancer; and a kit comprising same.