Micro Bio Sensor With Immobilized Antibiotics For Rapid Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional methods for detecting microorganisms in foods, drinks, and cosmetics are time-consuming and require skilled handling, with low sensitivity and high costs, making them unsuitable for rapid and concurrent detection of multiple species.
Innovation Solution
A micro bio sensor with a self-assembled monolayer and immobilized antibiotics on a detector, using three electrodes to detect microorganisms by changes in impedance, allowing for high sensitivity and rapid detection of multiple species.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If conventional agar medium cultivation method is used, then microbe detection can be performed, but detection time is excessively long (24 to 120 hours)
Solution Approach 1:
The patent replaces the biological cultivation system (agar medium, bacterial growth) with an electrochemical detection system. The microbe detection is achieved through electrochemical reactions at the electrode surface, where antibiotics immobilized on the electrode interact with microbes, producing detectable electrical signals. This substitution eliminates the need for lengthy biological cultivation while maintaining detection reliability through sensitive electrochemical measurement.
Solution Approach 2:
The patent changes the detection parameter from biological growth (colony counting after days) to electrochemical parameters (current, voltage, impedance changes). By measuring electrical properties rather than biological mass, the system achieves rapid detection within minutes to hours, dramatically reducing detection time while maintaining sensitivity through precise electrochemical measurement.
2Ease of operation
If conventional agar medium method is used, then microbe counting can be performed, but various skilled operations are required
Solution Approach 1:
The patent implements self-service by allowing the electrode to automatically detect microbes through electrochemical reactions. The immobilized antibiotics on the electrode surface passively interact with microbes in the sample, and the electrochemical signal is automatically generated and measured by the device. This eliminates the need for skilled manual operations such as medium preparation, incubation monitoring, and colony counting, enabling simple, automated detection.
Solution Approach 2:
The patent replaces complex mechanical and biological operations with automated electrochemical measurement. Instead of requiring skilled hands to prepare media, count colonies, and monitor growth, the system uses automated electrochemical sensors to detect and quantify microbes through electrical signals, dramatically simplifying operation and increasing throughput.
3Speed
If electrochemical reaction method is used, then detection speed is improved, but detection sensitivity and apparatus cost are problematic
Solution Approach 1:
The patent applies preliminary action by pre-immobilizing antibiotics on the electrode surface before detection. This preparation step ensures that the electrode is already optimized for specific microbe detection, enhancing sensitivity. The antibiotics are covalently bonded or adsorbed onto the electrode in advance, creating an active detection surface that immediately interacts with microbes in the sample, thereby achieving both rapid and sensitive detection.
Solution Approach 2:
The patent uses composite materials by combining the electrode with immobilized antibiotics and self-assembled monolayers. This composite structure integrates the electrochemical detection function with the biological recognition function. The combination of electrode material, antibiotic layer, and monolayer creates a multi-functional sensor that achieves high sensitivity through the synergistic interaction of different materials while maintaining rapid electrochemical response.
4Measurement precision
If crystal oscillator with antimicrobial antibody is used, then detection sensitivity is improved, but the method is not suitable for detecting multiple species concurrently
Solution Approach 1:
The patent achieves universality by designing a multi-functional electrode system that can detect multiple microbe species concurrently. The electrode surface is functionalized with various antibiotics that can recognize different microbe types, and the electrochemical measurement system can detect signals from all species simultaneously. This allows a single device to perform multiple detection functions, making it adaptable to various microbe species without requiring separate specialized sensors for each.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and sensitive detection of microorganisms, improving quality control by simplifying the detection process and reducing costs, while effectively identifying multiple species simultaneously.
Implementation Method 1
a self-assembled monolayer which is formed on the detector and an antibiotic which is immobilized through the self-assembled monolayer on the detector
Implementation Method 2
an antibiotic which is immobilized through the self-assembled monolayer on the detector
Implementation Method 3
a method for detecting a microbe based on changes in frequency that are caused by bonding the microbe to a surface of an electrode in a state that a voltage is applied between two electrodes
Data Source
AI summary
A micro bio sensor which detects a microbe existing in a specimen is provided. The microbial sensor includes a base, a detector formed on the base, and a reaction layer formed on the detector, wherein the reaction layer is comprised of a self-assembled monolayer which is formed on the detector and an antibiotic which is immobilized through the self-assembled monolayer on the detector. By using the micro bio sensor, it is possible to detect species of the microbe concurrently and improve sensitivity for detecting the species of the microbe. Further, a method for manufacturing such a micro bio sensor is also provided.