Microalgae PDRN Extraction for High Purity and Viral Safety
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for obtaining polydeoxyribonucleotide (PDRN) face challenges such as reliance on animal-derived sources, which can lead to contamination risks, seasonal extraction constraints, and potential viral infections, limiting scalability and safety.
Innovation Solution
A method for producing high-purity PDRN from microalgae, involving anionic surfactants, metal salts, and alcohol purifications, enabling mass cultivation and extraction without external constraints, ensuring safety and efficacy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If PDRN is extracted from animal-derived sources (fish semen and testes), then PDRN can be obtained with pharmacological activity, but there is a risk of viral contamination, zoonotic infections, and seasonal extraction constraints
Solution Approach 1:
The patent extracts PDRN from plant-based sources (microalgae, moss, higher plants) instead of animal-derived sources. This substitution removes the risk of viral contamination and zoonotic infections associated with animal tissues while maintaining the pharmacological activity of PDRN through its DNA polymer structure
Solution Approach 2:
The patent changes the source material parameter from animal tissue to plant material, fundamentally altering the biological origin while preserving the functional properties of PDRN. This parameter change eliminates the harmful factors associated with animal-derived PDRN
2Productivity
If PDRN is extracted from fish semen and testes, then PDRN can be mass-purified, but extraction is limited to seasonal cycles and may cause mass fish deaths
Solution Approach 1:
The patent uses plant-based materials (microalgae, moss, higher plants) that can be cultivated year-round through various methods including hydroponics and controlled environments. This provides universal availability regardless of season, enabling continuous PDRN production without the temporal constraints of fish-based extraction
Solution Approach 2:
The patent creates an alternative production system using plant materials that replicates the PDRN production capability without relying on fish species. This copying approach maintains productivity while eliminating seasonal and ethical constraints
3Quantity of substance
If PDRN is extracted from animal tissues, then DNA can be obtained for PDRN synthesis, but there is a possibility of contamination with exogenous viruses that may be zoonotic or human-transmissible
Solution Approach 1:
The patent converts the potential harm of viral contamination into a benefit by using plant-based sources that are inherently free from animal viruses. The plant origin becomes an advantage, providing DNA for PDRN synthesis without the risk of zoonotic or human-transmissible viruses
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method yields a high-purity PDRN extract with a DNA content of 49 wt% or more, primarily low molecular weight DNA, suitable for cosmetic and therapeutic applications, enhancing skin absorption and safety by avoiding viral risks.
Implementation Method 1
adding an anionic surfactant and a metal salt to a microalgae solution and stirring the resulting mixture to obtain a cytolysate
Implementation Method 2
adding a metal salt to a filtrate obtained by centrifuging and filtering the cytolysate of step (1) to stir the resulting mixture
Implementation Method 3
adding a C1-C4 alcohol to a filtrate obtained by centrifuging and filtering the stirred solution of step (2) and centrifuging the resulting mixture to obtain a precipitate
Implementation Method 4
freeze-drying the precipitate of step (4)
Data Source
AI summary
The present disclosure relates to a high-purity PDRN extract derived from microalgae and a preparation method thereof. Specifically, the high-purity PDRN extract derived from microalgae according to the present disclosure is easy to produce and highly sustainable by using microalgae capable of being mass-cultivated at any time without external environmental or time constraints as a raw material, is safe due to no risk of human infection by exogenous viruses, and can also be excellent in skin absorption when applied to products such as cosmetics due to its low molecular weight.