Microarray Primer Probe Sets for Respiratory Pathogen Detection

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Current methods lack primer sets capable of specifically amplifying and detecting target sequences of virulence factor genes from twelve respiratory disease-causing bacterial species, and probes specific to these sequences are not available for effective detection.

Innovation Solution

A primer set and probe set are designed to specifically amplify and detect target sequences from twelve respiratory disease-causing bacterial species by targeting specific virulence factor genes, including adeB, Tcf, Omp, tsx, rpoS, uspA, Wab, Mip, cop, P1, ETA, and lytA genes, using oligonucleotides immobilized on microarrays for hybridization.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional probes are used for detection, then detection capability is provided, but specificity to virulence factor genes of twelve bacterial species is insufficient

Engineering Contradiction:

Improvedetection specificityVSAvoiddetection coverage

Core Design Contradiction:

Measurement precisionVSAdaptability or versatility

Solution Approach 1:

The detection system is segmented into species-specific primer pairs, each targeting unique virulence factor genes of individual bacterial species. This segmentation enables simultaneous detection of multiple species with high specificity by dividing the detection function into separate, specialized components.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The invention creates a universal detection platform that can detect all twelve respiratory disease-causing bacterial species through a single microarray system containing multiple species-specific probes. This multi-functional system replaces the need for separate detection assays for each species.

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Measurement precision

If species-specific primer pairs are designed for each bacterial species, then detection specificity is improved, but system complexity increases

Engineering Contradiction:

Improvedetection specificityVSAvoidprimer set complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

Multiple species-specific primer pairs and probes are merged into a single microarray platform that can simultaneously detect all twelve bacterial species. This consolidation reduces operational complexity by allowing parallel detection of all species in one assay rather than requiring separate assays for each species.

Inventive Principle:

Principle #5Merging (Combining)

Solution Approach 2:

The primer pairs and probes are pre-designed and pre-optimized to target conserved regions of virulence factor genes across different species. This preliminary design phase ensures high specificity while maintaining manageable complexity by using standardized design criteria and validation protocols.

Inventive Principle:

Principle #10Preliminary action

3Productivity

If multiple bacterial species are detected simultaneously, then detection efficiency is improved, but false positive risk increases

Engineering Contradiction:

Improvedetection efficiencyVSAvoidfalse positive rate

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

Each probe on the microarray is designed with unique sequence characteristics and binding specificities tailored to its target species' virulence factor gene. This local optimization of probe quality ensures that each detection reaction is highly specific, reducing false positives even when multiple species are detected simultaneously in parallel.

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The solution enables specific and selective detection of the twelve bacterial species with high sensitivity and specificity, facilitating accurate identification of respiratory disease-causing pathogens.

Implementation Method 1

a primer set capable of specifically amplifying target sequence(s) of twelve respiratory disease-causing bacterial species

Methodology Applied

Scientific EffectPolymerase chain reaction:

Implementation Method 2

Two single strands of a nucleic acid comprised of nucleotides hybridize to form a double helical structure in which the two polynucleotide chains running in opposite directions are held together by hydrogen bonds between matched base pairs

Methodology Applied

Scientific EffectNucleic acid hybridization:

Implementation Method 3

a microarray on which the probe set is immobilized

Methodology Applied

Scientific EffectImmobilization:

Data Source

PatentUS7659388B2Method and compositions for detecting respiratory disease-causing bacterial species

Publication Date: 2010.02.09 SAMSUNG ELECTRONICS CO LTD

AI summary

Provided are a primer set for specifically amplifying target sequence(s) of twelve respiratory disease-causing bacterial species, a probe set specifically hybridizing with target sequence(s) of twelve respiratory disease-causing bacterial species, a microarray comprising the probe set, and a method of detecting a respiratory disease-causing bacterial species using the probe set.