Universal Microbead Detection via β2GPI and Oxidizing Metal Ions
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Solution Overview
Problem
Current methods for detecting and concentrating infectious compounds in biological materials face challenges such as interference from inhibitory substances and the need for virus-specific microbeads, which are not universally applicable due to varying virus properties.
Innovation Solution
The method involves loading microbeads with β2GPI proteins and using oxidizing metal ions like copper to ensure universal binding of infectious compounds, allowing for their concentration and detection without modifying the microbead polymer based on the virus type, and using these microbeads to capture and identify pathogens in biological samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If virus-specific microbeads are used for detection, then detection specificity is improved, but device complexity and manufacturing difficulty increase due to varying virus properties requiring different microbead compositions
Solution Approach 1:
The patent applies universality by using a single microbead composition (polyethylene-imine) that can detect multiple types of viruses through the intermediary作用 of β2GPI protein. Instead of creating virus-specific microbeads for each pathogen type, the invention uses one universal microbead formulation that works across different viruses via the protein intermediary mechanism, thereby reducing complexity while maintaining detection specificity.
Solution Approach 2:
The patent introduces β2GPI protein as an intermediary substance that bridges the microbead surface and viral compounds. The microbeads bind to β2GPI, which in turn binds to viral compounds, creating a detection cascade. This intermediary mechanism allows a single microbead type to detect multiple virus types without direct virus-specific binding, resolving the contradiction between specificity and complexity.
2Measurement precision
If β2GPI is added to capture infectious compounds, then detection sensitivity is improved by concentrating pathogens, but the presence of inhibitory substances in biological materials reduces detection accuracy
Solution Approach 1:
The patent extracts and removes inhibitory substances from the biological material through a purification step before the detection process. By separating and eliminating these interfering substances, the invention creates a cleaner sample matrix that allows β2GPI to effectively bind to infectious compounds without interference, thereby maintaining high detection sensitivity while overcoming the harmful effect of inhibitory substances.
3Measurement precision
If microbeads are modified based on virus type, then detection accuracy for specific pathogens is improved, but ease of manufacture decreases due to ad hoc preparation requirements
Solution Approach 1:
The patent implements a universal microbead formulation (polyethylene-imine) that does not require modification based on virus type. The same microbead composition works for all viruses when combined with β2GPI, eliminating the need for ad hoc preparation and standardizing manufacturing. This universal approach maintains detection accuracy across different pathogens while dramatically improving ease of manufacture.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively captures and detects infectious compounds, including viruses and bacteria, by forming a stable complex with β2GPI, enhancing detection sensitivity and universality across different pathogens, and allows for their identification through PCR or immunofluorescence.
Implementation Method 1
a loading of the microbeads in the suspension with β2GPI proteins is ensured by coupling with a sufficient quantity of β2GPI proteins
Implementation Method 2
in a container, said microbeads loaded with β2GPI proteins are brought into contact with the fluid medium M by adding ions of at least one oxidizing metal
Implementation Method 3
microbeads having a core formed by one (or more) particle(s) of magnetic material in order to allow their separation from the suspension medium using a magnetic field
Implementation Method 4
the infectious compounds are detected and/or quantified and/or identified, starting from the concentrated residue thus obtained
Implementation Method 5
a fluorescent or radiolabelled tracer
Implementation Method 6
a fluorescent or radiolabelled tracer
Data Source
AI summary
Method for in vitro detection and/or quantification and/or identification of infectious compounds present in a fluid medium M constituting a biological material, in which method a suspension of microbeads of solid polymer material capable of binding proteins is prepared; the microbeads are loaded with β2GPI proteins by coupling with a sufficient amount of β2GPI proteins; said microbeads are brought into contact with the fluid medium M while adding ions of at least one oxidizing metal, so as to bind the infectious compounds to the β2GPI proteins; the microbeads thus prepared are separated from their suspension medium, so as to obtain a residue; and the infectious compounds of the residue are detected and/or quantified and/or identified.