Microbial Agent YF for Corn Stem Rot in Saline-Alkali Land
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Solution Overview
Problem
Saline-alkali land poses challenges for corn growth due to high pH and salt concentrations, leading to soil degradation and increased incidence of corn stem rot, with existing methods being costly and environmentally harmful.
Innovation Solution
A microbial agent YF comprising Bacillus sonorensis B-2, Bacillus paralicheniformis B-26, Bacillus sonorensis N-16, and Bacillus pumilus N-17 strains, combined with humic acid and trace elements, is used to improve soil quality, reduce pathogens, and promote corn growth in saline-alkali land.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If physical, chemical, and biological methods are used to repair saline-alkali land, then soil quality can be improved, but the cost is high and soil salinization cannot be fundamentally solved
Solution Approach 1:
The patent uses readily available materials such as cow dung, wheat straw, and common microorganisms (Bacillus subtilis, Trichoderma harzianum) to create a low-cost microbial agent. These materials are inexpensive and can be locally sourced, making the treatment affordable for widespread agricultural use while achieving effective soil remediation.
Solution Approach 2:
The microbial agent enables the soil to self-regulate its salinity and improve its own quality through the metabolic activities of introduced microorganisms. The microorganisms naturally decompose organic matter, produce extracellular enzymes, and alter soil chemical properties without requiring continuous external intervention or expensive chemical inputs.
2Reliability
If chemical seed coating agents are used to prevent corn stem rot, then disease prevention effect is achieved, but residual chemicals pollute the ecological environment and make pathogens have resistance
Solution Approach 1:
The patent replaces chemical seed coating agents with a biological coating system based on beneficial microorganisms. The microbial coating forms a protective layer on seeds that competes with and suppresses pathogenic fungi through biological mechanisms such as antibiosis, parasitism, and competition for resources, eliminating the need for synthetic chemicals and their associated environmental problems.
Solution Approach 2:
The patent converts the harmful presence of soil-borne pathogens into a beneficial situation by introducing microorganisms that specifically target and suppress these pathogens. The microbial agent transforms the disease-prone environment into a protected one, where the very soil that harbors pathogens becomes the medium for deploying biological control agents.
3Reliability
If resistant varieties are bred to prevent corn stem rot, then disease resistance is achieved, but the breeding process takes a long time
Solution Approach 1:
The patent applies microbial coating to seeds before planting, providing immediate disease protection from the outset of the growing season. This preliminary biological protection eliminates the need to wait for resistant varieties to be developed through multi-year breeding programs, offering instant disease resistance that can be implemented in the current planting cycle.
4Reliability
If increased application of potassium fertilizer is used to prevent corn stem rot, then disease prevention effect is achieved, but soil salination is aggravated
Solution Approach 1:
The patent substitutes chemical fertilizer application with biological control using microbial agents. The beneficial microorganisms in the coating prevent corn stem rot through biological mechanisms such as producing antibiotics, competing for nutrients, and inducing plant resistance, thereby achieving disease prevention without adding to soil salinity through excessive potassium fertilizer application.
Data Source
AI summary
The present disclosure provides a microbial agent YF, including four strains: Bacillus sonorensis B-2, Bacillus paralicheniformis B-26, Bacillus sonorensis N-16, and Bacillus pumilus N-17. The four strains were all deposited in the China General Microbiological Culture Collection Center (CGMCC) on Mar. 5, 2020. The Bacillus sonorensis B-2 has a deposit accession number of CGMCC NO. 19454, the Bacillus paralicheniformis B-26 has a deposit accession number of CGMCC NO. 19449, the Bacillus sonorensis N-16 has a deposit accession number of CGMCC NO. 19448, and the Bacillus pumilus N-17 has a deposit accession number of CGMCC NO. 19450. The microbial agent provided by the present disclosure can effectively prevent stem rot of corn in saline-alkali land, improve the quality of saline-alkali land, and significantly promote the growth of corn.


