Microbial Biomass Composition for Sustainable Fish Feed

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Solution Overview

Problem

Current fish feed sources, such as fish meal and fish oils, are finite, expensive, and environmentally unsustainable, while alternative plant and animal by-products face consumer acceptance issues and do not adequately provide essential omega-3 fatty acids and amino acids, leading to oxidation and decomposition problems in microbial-based feeds.

Innovation Solution

A microbial biomass composition with 15-95% protein and 25-55% DHA, grown through fermentation, is developed to provide a cost-effective, sustainable source of protein and omega-3 fatty acids, using Thraustochytrids like Schizochytrium mangrovei, which reduces oxidation risks and meets fish nutritional needs.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If fish meal and fish oils are used as feed sources, then protein and omega-3 fatty acid content is high, but cost is high and environmental sustainability is poor

Engineering Contradiction:
Improveprotein and omega-3 contentVSAvoidcost and sustainability
Core Design Contradiction:
Quantity of substanceVSEase of manufacture

Solution Approach 1:

The patent changes the source parameter from marine animals to microorganisms (Thraustochytrids), fundamentally altering how protein and omega-3 fatty acids are produced. This microbial fermentation approach maintains high nutritional content while reducing dependency on finite fish resources, thereby lowering cost and improving sustainability

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent employs microorganisms that can be rapidly cultivated and harvested through fermentation processes. These microbial cells serve as a renewable, cost-effective alternative to expensive fish meal, allowing continuous production without depleting marine ecosystems

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

2Ease of manufacture

If plant byproducts are used as alternative protein sources, then cost is reduced, but essential amino acid content is insufficient

Engineering Contradiction:
Improvecost effectivenessVSAvoidessential amino acid content
Core Design Contradiction:
Ease of manufactureVSQuantity of substance

Solution Approach 1:

The patent transitions from plant-based protein sources to microbial protein sources, changing the biochemical composition parameters. Thraustochytrid biomass naturally contains all essential amino acids in balanced proportions, matching or exceeding fish meal quality while maintaining cost advantages over traditional animal feeds

Inventive Principle:
Principle #35Parameter changes

3Ease of manufacture

If microbial biomass is used as feed source, then sustainability and cost-effectiveness improve, but oxidation and decomposition of fatty acids occur

Engineering Contradiction:
Improvecost-effectiveness and sustainabilityVSAvoidoxidation stability
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent applies preliminary preservation actions by incorporating antioxidants during the microbial biomass production and processing stages. This preventive approach protects omega-3 fatty acids from oxidation before the feed reaches consumers, maintaining nutritional quality and safety

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent introduces antioxidant substances as intermediary protective agents between the omega-3 rich microbial biomass and environmental oxidants. These antioxidants act as mediators that sacrifice themselves to prevent oxidation of the valuable fatty acids, ensuring feed stability

Inventive Principle:
Principle #24Intermediary (Mediator)

4Quantity of substance

If DHA content in microbial biomass is increased, then nutritional value for fish improves, but susceptibility to oxidation increases

Engineering Contradiction:
ImproveDHA contentVSAvoidoxidation susceptibility
Core Design Contradiction:
Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The patent implements preliminary protective measures by incorporating antioxidants during biomass production and processing. This advance protection is particularly important for high-DHA strains, creating a stable product that maintains both high nutritional value and oxidation resistance throughout storage and feeding periods

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The microbial biomass composition offers a cost-effective, sustainable, and nutritionally balanced feed for fish, reducing production costs and oxidation risks, while ensuring high DHA content and meeting consumer protein requirements, thus addressing the limitations of existing fish feed sources.

Implementation Method 1

the microbial biomass is grown by fermentation for less than 4 days, preferably less than 2 days, at 25-39°C to produce a concentration of microbial biomass in the range of 100-350 g/l based on dry cell weight

Methodology Applied
Scientific EffectFermentation: Fermentation

Data Source

PatentEP3570690A1Composition and method of production thereof
Publication Date: 2019.11.27 HUVE NUTRACEUTICALS LTD

AI summary

The invention relates to food compositions comprising microbial biomass, the microbial biomass comprising:(a) 15-95 dry cell wt% protein; and(b) 25-55% DHA as a percentage of total fatty acids. The present invention also relates to a method for growing microbial biomass by fermentation at 25-39°C, for less than 4 days resulting in a yield of 100-350 g/l.