Microbial Cellular Biomass Production for Low-Impact Flocculation

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Solution Overview

Problem

Water treatment plants rely heavily on synthetic polymers like polyacrylamide, which are costly, environmentally harmful, and pose health risks, while biopolymers like bacterial exopolymers are underutilized due to high production costs and inefficiencies, necessitating a cost-effective, environmentally friendly alternative for flocculation processes.

Innovation Solution

A process for producing microbial cellular biomass with flocculant properties using a substrate rich in monosaccharides, controlled C/N and C/P ratios, and selective decantation to eliminate undesirable microorganisms, resulting in a biomass that can replace synthetic polymers.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If synthetic polymers like polyacrylamide are used for flocculation, then flocculation efficiency is improved, but environmental harm and health risks increase

Engineering Contradiction:
Improveflocculation efficiencyVSAvoidenvironmental harm and health risks
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent changes the chemical composition parameters by replacing synthetic polyacrylamide with microbial cellular biomass having different biochemical properties. The biomass contains exopolysaccharides and proteins that provide flocculation functionality while eliminating the harmful effects of synthetic polymers, thus resolving the contradiction between efficiency and environmental safety

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent uses microbial biomass that can be produced locally and is biodegradable, replacing persistent synthetic polymers. The biomass serves its flocculation function and then degrades naturally, eliminating long-term environmental accumulation and health risks associated with synthetic materials

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

2Object-affected harmful factors

If biopolymers like bacterial exopolymers are used to replace synthetic polymers, then environmental friendliness is improved, but production cost increases

Engineering Contradiction:
Improveenvironmental impactVSAvoidproduction cost
Core Design Contradiction:
Object-affected harmful factorsVSEase of manufacture

Solution Approach 1:

The patent employs mixed microbial cultures that self-organize and self-produce the necessary exopolysaccharides and flocculant substances without requiring complex external intervention. The system uses inexpensive substrates and maintains itself through natural microbial interactions, significantly reducing production costs compared to controlled pure culture systems

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent uses mixed microbial cultures that perform multiple functions simultaneously: substrate degradation, biomass production, and exopolysaccharide secretion. This multi-functionality eliminates the need for separate processing steps and reduces overall production costs while maintaining environmental friendliness

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Reliability

If pure microbial cultures are used for EPS production, then flocculant properties are improved, but production complexity and cost increase

Engineering Contradiction:
Improveflocculant propertiesVSAvoidproduction process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

Instead of using pure cultures and attempting to extract EPS, the patent inverts the approach by directly using the entire microbial cellular biomass including cells and extracellular substances. This inversion simplifies the production process while maintaining or improving flocculant properties, as the complete biomass provides both cellular and extracellular flocculation mechanisms

Inventive Principle:
Principle #13The other way round (Inversion)

4Reliability

If conventional clarification processes using chemical coagulants are used, then treatment effectiveness is improved, but chemical consumption and environmental impact increase

Engineering Contradiction:
Improvetreatment effectivenessVSAvoidchemical consumption
Core Design Contradiction:
ReliabilityVSLoss of substance

Solution Approach 1:

The patent extracts and utilizes the natural flocculant capabilities inherent in microbial biomass, replacing the need for added chemical coagulants. The microbial cells and their extracellular substances provide the coagulation and flocculation functions that would otherwise require separate chemical additives, thereby reducing chemical consumption while maintaining treatment effectiveness

Inventive Principle:
Principle #2Taking out (Extraction)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The produced microbial biomass achieves flocculation efficiency comparable to or exceeding that of synthetic polymers, reducing chemical usage and environmental impact, and can be used in various aqueous and oily solutions.

Implementation Method 1

Bacterial EPS, and in particular the fraction comprising exopolysaccharides, may present for some, excellent flocculating properties and may thus be a satisfactory alternative to synthetic polymers such as PAM

Methodology Applied
Scientific EffectFlocculation: Flocculation

Implementation Method 2

elimination of any filamentous bacteria and filamentous fungi by sequential decantation of the obtained biomass

Methodology Applied
Scientific EffectSedimentation: Sedimentation

Data Source

PatentUS12459842B2Method for producing microbial cellular biomass having flocculant properties
Publication Date: 2025.11.04 INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE
  • US12459842B2 patent drawing
  • US12459842B2 patent drawing
  • US12459842B2 patent drawing

AI summary

The disclosure relates to a microbial cellular biomass having flocculant properties, to its obtaining process and to its use in the treatment by flocculation.