Microbial Cytoplasmic Extract Purification at Low Temperature
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Solution Overview
Problem
Existing methods for producing microbial cytoplasmic macromolecular extracts fail to effectively decolorize, deodorize, and desalinate while maintaining biological activity, leading to quality issues in cosmetic applications due to color, odor, and inorganic salts.
Innovation Solution
A multi-step process involving dilution, separation, low-temperature cell wall breaking, microfiltration, and high-speed centrifugation to produce a pure, colorless, odorless, and salt-free microbial cytoplasmic macromolecular extract that retains biological activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If traditional cell wall breaking technology (autolysis and enzyme hydrolysis) is used, then the microbial cytoplasm can be extracted, but the biological activity of microbial cytoplasm macromolecular substances is destroyed
Solution Approach 1:
The patent changes the physical parameters of cell wall breaking by using high-pressure homogenization (100-200 MPa) instead of traditional enzymatic or autolytic methods. This physical parameter change allows cell wall disruption while preserving the biological activity of macromolecular substances within the cytoplasm.
Solution Approach 2:
The patent replaces chemical/biological cell wall breaking methods (enzymes, autolysis) with a mechanical system (high-pressure homogenization). This substitution achieves cell wall disruption through mechanical force while avoiding the degradation of biological molecules that occurs with enzymatic hydrolysis.
2Quantity of substance
If microbial cytoplasm is used directly in cosmetics, then macromolecular nutrients are provided, but color and odor substances and inorganic salts affect the quality of cosmetics
Solution Approach 1:
The patent extracts and removes harmful substances (color, odor, inorganic salts) from the microbial cytoplasm extract through a series of purification steps including high-speed centrifugation and filtration, while retaining the beneficial macromolecular nutrients for cosmetic application.
Solution Approach 2:
The patent segments the microbial cytoplasm components by separating macromolecular substances from medium and small molecular substances through controlled filtration and centrifugation, allowing selective removal of harmful small molecules while preserving beneficial macromolecules.
3Object-affected harmful factors
If multiple purification steps are added to remove color, odor, and salts, then cosmetic quality improves, but the complexity of the production process increases
Solution Approach 1:
The patent combines multiple purification functions (cell wall breaking, cell lysis, separation, and purification) into an integrated production flow where each step serves multiple purposes. For example, high-pressure homogenization both breaks cell walls and releases cytoplasmic contents, while high-speed centrifugation simultaneously separates macromolecules from small molecules.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process ensures the production of a high-quality microbial cytoplasmic macromolecular extract suitable for cosmetics, maintaining biological activity and removing impurities, thereby enhancing its functional effectiveness in skincare applications.
Implementation Method 1
filtering the obtained liquid containing microbial cytoplasm substances by a microfiltration membrane to remove medium and small molecular substances
Implementation Method 2
separating the obtained pure microbial cytoplasm macromolecular substance concentrate by a high-speed centrifuge to remove solid substances
Data Source
Figure 1
AI summary
The present invention discloses a process for producing microbial cytoplasm macromolecular extract. The present invention uses microorganisms as raw materials and adopts a low-temperature cell wall breaking method to break the microbial cell wall. The soluble substances in the microbial cytoplasm are released by breaking the cell wall. Then, the solid substances in the cell wall breaking mixture are separated to obtain the soluble substances in the microbial cytoplasm. Then, the medium and small molecular substances containing color, odor and inorganic salts in the soluble substances are removed by microfiltration to obtain concentrated macromolecular substances. The medium and small molecular substances are further removed by repeated dilution with water and then filtration and concentration to obtain a pure colorless, odorless and salt-free cytoplasm macromolecular extract. During the entire production process, a low temperature is always maintained and no other chemical substances and enzymes are added, which ensures that the obtained microbial cytoplasm macromolecular extract maintains its original biological activity.