Microbial Sensor Electrode Configuration for Soil Biochemical Monitoring

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Solution Overview

Problem

Existing microbial sensor systems are not suitable for monitoring soils, especially the rhizosphere and contaminated soils, nor can they directly measure biochemical characteristics of plants, limiting their effectiveness in assessing environmental health and remediation efforts.

Innovation Solution

A microbial sensing system comprising an indicator electrode, a reference electrode, and a signal acquisition module with high-impedance potentiometry, capable of measuring open-circuit voltage and recovery voltage, is used to characterize biochemical conditions in soils and plant tissues, allowing for the investigation of microbial activity, substrate concentrations, and other biochemical characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional microbial sensor systems are used, then energy conversion from waste products is achieved, but the systems are not suitable for monitoring soils and plant tissues

Engineering Contradiction:
Improvesuitability for monitoring soils and plant tissuesVSAvoideffectiveness in assessing environmental health
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The sensor system is designed with different electrode configurations and materials optimized for specific measurement environments. Indicator electrodes can be made from graphite, gold, platinum, titanium, or carbon fabrics, and the system can operate in different modes (open-circuit voltage, recovery voltage, controlled current) depending on whether it is monitoring soil, sediment, or plant tissue, allowing each local application to have the appropriate sensor characteristics

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The microbial sensor system is designed to perform multiple functions across different environments. It can monitor biochemical characteristics in soils, sediments, and plant tissues using the same basic electrochemical platform, eliminating the need for environment-specific sensor designs and enabling universal application for environmental health assessment

Inventive Principle:
Principle #6Universality (Multi-functionality)

2Measurement precision

If conventional sensors are used in aqueous environments, then biochemical conditions can be determined, but the sensors cannot directly measure biochemical characteristics of plants

Engineering Contradiction:
Improvedirect measurement of plant biochemical characteristicsVSAvoidcapability to measure in plant tissues
Core Design Contradiction:
Measurement precisionVSAdaptability or versatility

Solution Approach 1:

The system changes its operational parameters when measuring plant tissues versus soils. It can switch between open-circuit voltage mode, recovery voltage mode, and controlled current mode depending on the measurement target, and adjusts electrode materials and configurations to optimize for the specific electrical and biochemical properties of plant tissues

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The system uses indicator electrodes that can directly contact plant tissues, serving as intermediaries between the measurement system and the plant's internal biochemical environment. These electrodes facilitate direct electrical contact with plant cells and tissues, enabling measurement of internal plant biochemical characteristics without requiring tissue extraction or destruction

Inventive Principle:
Principle #24Intermediary (Mediator)

3Measurement precision

If high-impedance potentiometry is used, then open-circuit voltage measurements are achieved, but device complexity increases

Engineering Contradiction:
Improveopen-circuit voltage measurement accuracyVSAvoidsignal acquisition module complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The system replaces complex mechanical measurement systems with electrochemical sensing. Instead of using complex physical measurement devices, it uses electrochemical cells with indicator and reference electrodes that naturally generate electrical signals through biochemical reactions, simplifying the overall measurement system while maintaining high precision

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables effective monitoring of rhizosphere and plant health by providing detailed biochemical data, including microbial compositions and disease detection, facilitating environmental assessments and remediation strategies.

Implementation Method 1

signal acquisition module electrically coupled to the indicator electrode and the reference electrode, wherein the signal acquisition module comprises measurement circuitry that comprise a high-impedance potentiometer, having an impedance greater than or equal to 10 megaohms

Methodology Applied
Scientific EffectHigh-impedance potentiometry: Electrical Resistance

Implementation Method 2

measuring a potential difference between the indicator electrode and a reference electrode

Methodology Applied
Scientific EffectElectrochemical potential difference: Electromagnetic Induction

Data Source

PatentUS11226306B2Microbial sensor system for monitoring and imaging of an environment
Publication Date: 2022.01.18 BURGE ENVIRONMENTAL INC
  • US11226306B2 patent drawing
  • US11226306B2 patent drawing
  • US11226306B2 patent drawing

AI summary

A microbial sensor, microbial sensing system, and method that can be used to determine the chemical environment of unsaturated soils, rhizosphere, and/or plants are disclosed. The microbial sensing system can be used for monitoring the health of plants including nutrients, salinity, contaminants, chemicals (pesticides, herbicides) and diseases. A microbial sensing system can include one or more indicator electrodes and a reference electrode. The microbial sensing system can include a signal acquisition and/or communication module to allow the real-time collection of data from field deployments and laboratory investigations.