Intestinal Microbiota Capsule Composition for Viability and Storage Stability
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Solution Overview
Problem
Current methods for preparing intestinal microbiota pellets, such as freeze-drying, result in reduced microbial viability, structural changes, and limited ambient-temperature stability, posing challenges for clinical deployment and patient safety.
Innovation Solution
A composition and method involving an eluent with plant extracts and Magnolia officinalis powder, combined with a protective agent and separation aid, to preserve microbial viability and enhance storage stability, using a device with specific filtration and centrifugation steps.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If freeze-drying treatment is used to prepare intestinal microbiota products, then the preparation process is simplified, but microbial viability is significantly reduced and microbial structure changes occur
Solution Approach 1:
The patent changes the preparation parameters from freeze-drying to a novel extraction method using specific eluents (buffer solution with pH 7.0-7.5) and controlled temperature (4°C-37°C), which preserves microbial viability while maintaining processability
Solution Approach 2:
The patent introduces a protective agent system comprising multiple components (protective polysaccharide, protective protein, protective enzyme) that act as intermediaries to protect microorganisms during the extraction and preparation process, preventing damage while enabling simplified processing
2Reliability
If microbiota pellet is prepared by separating undigested food residues and metabolites, then microbial viability is preserved, but the preparation process becomes more complex and ambient-temperature stability is limited
Solution Approach 1:
The patent extracts and removes harmful components (undigested food residues, metabolites, and other contaminants) through a systematic filtration and centrifugation process, isolating the microbial community while simplifying the overall preparation workflow
Solution Approach 2:
The patent creates a composite protective system combining multiple protective agents (polysaccharide, protein, enzyme) that work synergistically to maintain microbial viability and enhance stability, reducing the need for complex separate processing steps
3Stability of the object's composition
If cryogenic storage at -80°C is used, then storage stability is improved, but ice crystals form and pierce bacterial cell walls causing cell death
Solution Approach 1:
The patent applies protective agents (particularly protective polysaccharide and protective protein) beforehand to form a protective barrier around bacterial cells before cryogenic storage, cushioning them against ice crystal damage and maintaining viability during storage
Solution Approach 2:
The patent modifies the storage parameters by adding protective agents that change the freezing behavior of the microbial community, preventing ice crystal formation or reducing their harmful effects while maintaining low-temperature storage stability
4Ease of manufacture
If improper extraction methods are used, then preparation is simplified, but adverse effects occur on microbial viability and viable bacterial load increase
Solution Approach 1:
The patent optimizes extraction parameters including eluent composition (buffer solution with specific pH 7.0-7.5), temperature (4°C-37°C), and processing time to achieve simple yet effective extraction that preserves microbial viability and increases viable bacterial load
Solution Approach 2:
The patent introduces protective agents as intermediaries during the extraction process that facilitate the separation of microbial community from contaminants while protecting microorganisms from damage, enabling simplified extraction without compromising viability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves low-odor, high-viability intestinal microbiota pellets with improved storage stability, maintaining taxonomic abundance and viability for precise transplantation.
Implementation Method 1
using a device with specific filtration and centrifugation steps
Implementation Method 2
using a device with specific filtration and centrifugation steps
Implementation Method 3
combined with a protective agent and separation aid, to preserve microbial viability and enhance storage stability
Implementation Method 4
A composition and method involving an eluent with plant extracts and Magnolia officinalis powder
Data Source
AI summary
An intestinal microbiota product, a capsule, a preparation method and device therefore, and use thereof in precise microbiota transplantation are provided, relating to the technical field of intestinal microbiota transplantation. The composition provided by the present disclosure at least includes an eluent and a protective agent. The eluent includes a plant extract and Magnolia officinalis powder at a specific mass ratio, and the protective agent includes a small-molecular polyhydroxy compound, a macromolecular multipolymer, an amine substance, a natural emulsifier and an optional carbonate at a specific mass ratio. The two together constitute an organic whole.