Microchamber Array With Stacked Resin Layers for Fluorescence Detection
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Solution Overview
Problem
Existing microchamber arrays face issues with hydrophobic and hydrophilic solvents not being satisfactorily separated, leading to interference from resin autofluorescence during fluorescent analysis.
Innovation Solution
A microchamber array design with a stacked first and second resin layer configuration, where the second resin layer has lower autofluorescence and higher light transmittance, and the first resin layer is more hydrophobic, ensuring effective solvent separation and reduced autofluorescence interference.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If a single resin material is used for the chamber body, then the manufacturing process is simple, but hydrophobic and hydrophilic solvents cannot be satisfactorily separated
Solution Approach 1:
The chamber body is constructed using composite resin materials with different hydrophobicity levels. Specifically, a first resin layer (more hydrophobic) and a second resin layer (less hydrophobic) are stacked to form the chamber body, enabling effective separation of hydrophobic and hydrophilic solvents while maintaining manufacturing feasibility through layer-by-layer construction
2Ease of manufacture
If resin material is used for the chamber body, then the structure is easy to manufacture, but autofluorescence interferes with fluorescent analysis
Solution Approach 1:
The chamber body employs a composite resin structure where a second resin layer with low autofluorescence properties is stacked on a first resin layer. This composite configuration reduces overall autofluorescence interference during fluorescent analysis while maintaining the manufacturing advantages of resin materials
Solution Approach 2:
Different regions of the chamber body are assigned different resin materials with specific properties. The second resin layer (facing the observation side) is selected to have low autofluorescence, while the first resin layer provides structural support and hydrophobicity, creating local optimization of material properties for different functional requirements
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances the sensitivity and accuracy of fluorescent detection by minimizing autofluorescence from the resin layers, allowing for efficient separation and detection of hydrophobic and hydrophilic solvents.
Implementation Method 1
a contact angle of the first resin layer to water is larger than a contact angle of the second resin layer to water
Implementation Method 2
The chamber body includes a first resin layer and a second resin layer... contact angle of the first resin layer to water is larger than a contact angle of the second resin layer to water
Implementation Method 3
autofluorescence generated when the second resin layer is irradiated with excitation light is smaller than autofluorescence generated when the first resin layer is irradiated with excitation light
Data Source
AI summary
According to an aspect, a microchamber array includes: a first substrate; a second substrate facing the first substrate; a flow channel provided between the first substrate and the second substrate, a solvent containing a sample flowing through the flow channel; a chamber body provided on a surface of the first substrate facing the second substrate; and a plurality of storage portions provided in the chamber body. The chamber body includes a first resin layer and a second resin layer, the second resin layer and the first resin layer are stacked in this order on the first substrate, autofluorescence generated when the second resin layer is irradiated with excitation light is smaller than autofluorescence generated when the first resin layer is irradiated with excitation light, and a contact angle of the first resin layer to water is larger than a contact angle of the second resin layer to water.