Microcrop Protein Extraction System with Filtration and Dewatering

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Solution Overview

Problem

Current methods for extracting proteins from alternative sources face challenges due to inferior amino acid profiles, high fiber content, and unsuitable protein integrity, solubility, and dispersibility for animal and plant feed applications, while also considering water conservation concerns, especially in arid regions.

Innovation Solution

A system for recovering highly soluble protein from microcrops like Lemna using a series of processing units including lysing, separating, filtration, and drying, which includes microfiltration, ultrafiltration, and nanofiltration to achieve a protein concentrate with high solubility and dispersibility values.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If alternative plant species are used as protein sources, then production cost is reduced, but protein integrity, solubility, and dispersibility become unsuitable for feed applications

Engineering Contradiction:
Improveproduction costVSAvoidprotein integrity, solubility, and dispersibility
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent applies parameter changes by controlling pH levels during extraction (adjusting to isoelectric points of target proteins), temperature management, and using specific salt concentrations to optimize protein solubility and integrity. These parameter adjustments ensure that extracted proteins from alternative sources maintain functional properties suitable for feed applications while keeping production costs lower than marine sources.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces traditional mechanical extraction methods with electrostatic precipitation and electric field-based separation techniques. This substitution improves protein integrity by avoiding mechanical shear forces that could damage protein structures, while maintaining cost-effectiveness through energy-efficient electric field applications rather than expensive mechanical processing.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If marine protein sources are used, then desirable nutritional profile and palatability are achieved, but production costs increase

Engineering Contradiction:
Improvenutritional profile and palatabilityVSAvoidproduction cost
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent uses intermediary substances such as pH buffers, salts, and binding agents during the extraction process to bridge the gap between alternative plant proteins and the nutritional profile of marine proteins. These intermediaries help enhance amino acid availability and palatability of alternative protein sources, making them comparable to marine proteins while avoiding the high production costs associated with marine sourcing.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent modifies extraction parameters including pH, temperature, and pressure to optimize the nutritional profile of alternative protein sources. By adjusting these parameters, the extraction process maximizes amino acid content and bioavailability, achieving nutritional equivalence to marine proteins at lower production costs.

Inventive Principle:
Principle #35Parameter changes

3Device complexity

If traditional protein extraction methods are used, then production is simplified, but water consumption increases which is problematic in arid regions

Engineering Contradiction:
Improveprocess simplicityVSAvoidwater consumption
Core Design Contradiction:
Device complexityVSLoss of substance

Solution Approach 1:

The patent implements continuous extraction processes where water is recirculated through multiple extraction stages rather than being consumed in discrete batches. This continuous system maintains process simplicity while significantly reducing overall water consumption, making protein production viable in water-scarce arid regions by eliminating the need for repeated water addition and disposal.

Inventive Principle:
Principle #20Continuity of useful action

Solution Approach 2:

The patent recovers and reuses water from the extraction process through filtration and concentration stages. Water that would traditionally be discarded is instead recovered, cleaned, and fed back into the extraction system, thereby maintaining simple operational procedures while dramatically reducing net water consumption for protein production in arid environments.

Inventive Principle:
Principle #34Discarding and recovering

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The system effectively produces a protein concentrate with a protein concentration of at least 50% by weight, high solubility, and dispersibility, addressing the limitations of existing methods and providing a sustainable alternative for protein sources.

Implementation Method 1

a lysing unit to lyse a first portion of the biomass to form a first portion of lysed biomass

Methodology Applied
Scientific EffectLysis:

Implementation Method 2

a separating unit to separate the first portion of lysed biomass into a first portion of a juice fraction and a first portion of a solid fraction

Methodology Applied
Scientific EffectSeparation:

Implementation Method 3

a filtration unit (e.g., a microfiltration module) to filter the first portion of the first juice into a first portion of a soluble protein and a first reject stream

Methodology Applied
Scientific EffectMicrofiltration: Filter (physical)

Implementation Method 4

a filtration unit (e.g., an ultrafiltration module, a diafiltration module, a nanofiltration module) to filter the first portion of the soluble protein and generate a first portion of a second soluble protein and a second reject stream

Methodology Applied
Scientific EffectUltrafiltration: Semipermeable Membrane

Implementation Method 5

a filtration unit (e.g., an ultrafiltration module, a diafiltration module, a nanofiltration module) to filter the first portion of the soluble protein

Methodology Applied
Scientific EffectNanofiltration: Semipermeable Membrane

Implementation Method 6

a dewatering unit (e.g., a reverse osmosis filtration module, a nanofiltration module) to concentrate the first portion of the second soluble protein

Methodology Applied
Scientific EffectReverse Osmosis: Reverse Osmosis

Implementation Method 7

a drying unit to dry the first portion of the concentrated soluble protein and generate a first portion of a dry protein concentrate

Methodology Applied
Scientific EffectDrying: Evaporation

Data Source

PatentUS11166476B2Methods and systems for extracting protein and carbohydrate rich products from a microcrop and compositions thereof
Publication Date: 2021.11.09 LEMNA PRO LTD
  • US11166476B2 patent drawing
  • US11166476B2 patent drawing
  • US11166476B2 patent drawing

AI summary

The present disclosure relates, according to some embodiments, to systems for purifying proteins and carbohydrate rich products from photosynthetic aquatic species and compositions thereof. In some embodiments, a system for recovering a highly soluble protein product from a biomass comprising a microcrop (e.g., Lemna) may comprise (a) a lysing unit to lyse a first portion of the biomass to form a first portion of lysed biomass, (b) a first separating unit to separate the first portion of lysed biomass to generate a first portion of a juice fraction and a first portion of a solid fraction, (c) a second separating unit to separate the first portion of the juice fraction to generate a first portion of a first juice and a first portion of a first cake, (d) a first filtration unit to filter the first portion of the first juice to generate a first portion of a soluble protein and a first reject stream, (e) a second filtration unit to filter the first portion of the soluble protein to generate a first portion of a second soluble protein and a second reject stream, (f) a dewatering unit to concentrate the first portion of the second soluble protein to generate a first portion of a concentrated soluble protein, and (g) a drying unit to dry the first portion of the concentrated soluble protein to generate a first portion of a dry protein concentrate.