Microfluidic Analysis Instrument with Automated Sample Loading
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Solution Overview
Problem
Current analysis instruments for biological and biochemical samples are time-consuming, labor-intensive, and require significant reagent handling, with limitations in miniaturization, automation, and cost-effectiveness, particularly in slab gel electrophoresis processes.
Innovation Solution
A microfluidic analysis instrument with integrated sample storage, loading, and detection capabilities, featuring a moveable sample loading mechanism, automatic needle changeover, and a compact design that minimizes user intervention and reagent usage, enabling rapid and efficient processing of multiple samples in parallel.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional slab gel electrophoresis is used, then separation of charged molecules can be achieved, but the process is time-consuming and labor-intensive
Solution Approach 1:
The traditional slab gel electrophoresis process is divided into discrete automated steps: sample loading via automated pipetting, electrophoresis execution, and image capture. This segmentation enables each step to be optimized and automated independently, reducing overall process time while maintaining separation effectiveness.
Solution Approach 2:
The system performs self-service through automated sample loading mechanisms and integrated image capture. The automated pipetting system loads samples without manual intervention, and the system automatically captures images of the gel bands, eliminating time-consuming manual operations and accelerating the analysis process.
2Quantity of substance
If traditional gel processing is used, then molecular separation can be performed, but significant amounts of reagents are required and handling is complex
Solution Approach 1:
The essential function of gel electrophoresis is extracted and concentrated into a compact integrated system. The gel matrix, electrodes, and sample loading apparatus are combined in a single unit that requires minimal reagent handling. The system extracts only the necessary components for separation, eliminating the need for extensive buffer preparations and multiple separate handling steps.
Solution Approach 2:
The integrated apparatus performs multiple functions within a single device: it serves as the gel tank, sample loading station, power supply interface, and image capture system. This multi-functionality reduces the number of separate reagent containers and handling procedures required, simplifying the overall process while maintaining separation capabilities.
3Productivity
If automated sample loading is implemented, then processing efficiency improves, but device complexity increases
Solution Approach 1:
The automated sample loading mechanism is merged with the gel tank and power supply system into a single integrated unit. The automated pipetting apparatus combines the sample loading function with the electrophoresis chamber, eliminating the need for separate automated loading devices. This merging reduces overall system complexity while maintaining high processing efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The instrument achieves high-throughput analysis with reduced reagent and sample quantities, minimizing handling and costs, while maintaining a small footprint and ensuring efficient, automated processing of biological samples.
Implementation Method 1
gel bath based electrophoresis. This process enables separation of a complex mixture of charged molecules, such as nucleic acids or proteins, according to their electro-phoretic mobility
Implementation Method 2
detection means for detecting and/or measuring the reaction
Data Source
AI summary
An analysis instrument for processing a microfluidic device, having sample storage means, a microfluidic device holder, sample loading means for loading sample into a microfluidic device disposed in the holder, processing means for enabling a reaction in a microfluidic device, and detection means for detecting and/or measuring the reaction is disclosed. The microfluidic device holder is adapted to hold the microfluidic device including a tape in position for processing and/or detection. A microfluidic processing device is also disclosed which includes a reaction chamber, and a sample loading chamber into which a sample is injectable. The reaction chamber is operatively connected to the sample loading chamber. A cover extends across at least part of the sample loading chamber. The cover and the reaction chamber include pierceable material and are separated by an overspill cavity configured to accept any overspill of an injected sample. A kit is also disclosed which has the analysis instrument and the microfluidic processing device as described above.