Microfluidic Card Segmentation for Blood Sample Preparation
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Solution Overview
Problem
Proteomic analysis based on mass spectrometry for detecting and dosing proteins in biological samples is hindered by the variability in sample stability due to the time gap between blood sample collection, preparation, and analysis, leading to inconsistent results.
Innovation Solution
A micro-fluidic card with a compact and optimized fluidic circuit, including pneumatic control elements and extensible tanks, that performs all stages of blood sample preparation efficiently, minimizing elements and ensuring robustness and quality.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a standard micro-fluidic card is used to implement all preparation stages, then the device can perform complete blood sample preparation, but the fluidic circuit becomes complex and bulky
Solution Approach 1:
The micro-fluidic card is divided into distinct functional modules: a first chamber for blood sampling, a second chamber for plasma separation, and a third chamber for protein depletion/enrichment. Each chamber is connected through controlled fluidic pathways, allowing complete preparation functionality while maintaining modular simplicity and reducing overall circuit complexity.
2Adaptability or versatility
If multiple fluidic elements (valves, pumps, channels) are used to implement preparation stages, then all preparation steps can be performed, but the device becomes less robust and more complex
Solution Approach 1:
Multiple fluidic elements are merged into integrated components. The first, second, and third chambers are connected through a unified fluidic network with centralized control mechanisms, reducing the number of discrete valves and pumps while maintaining the ability to perform all preparation stages. This integration enhances robustness by reducing connection points and potential failure modes.
3Device complexity
If the fluidic circuit is optimized for compactness, then the device becomes more robust and simpler, but it becomes difficult to implement all preparation stages efficiently
Solution Approach 1:
The micro-fluidic card utilizes multi-dimensional space arrangement, stacking chambers and fluidic pathways in three-dimensional configuration rather than linear expansion. This allows compact integration of all preparation stages within a small footprint, maintaining both simplicity and efficiency through vertical and lateral integration of functional elements.
Data Source
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AI summary
The invention relates in particular to a microfluidic device comprising a rigid support element and a reservoir with adjustable volume made in said support, said reservoir (Rx) comprising: - A cavity (10) made in said support, said cavity comprising at least one opening leading onto a surface of said support, - At least one orifice (11) leading into the interior of said cavity, - A first flexible membrane (20) arranged to close said opening, - A functionalized developed surface (B), formed of a reagent or coated with a reagent, placed in said cavity of the reservoir with adjustable volume.