Microfluidic Detection Chip with Slope Drainage and Magnetic Transport
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Solution Overview
Problem
Current microfluidic detection chips face challenges such as interference from whole blood filtration, sample waste, and complex assembly processes, which affect detection sensitivity and precision, particularly in clinical point-of-care settings for high-sensitivity biomarkers like procalcitonin, N-terminal pro-brain natriuretic peptide, and troponin I.
Innovation Solution
A self-driving and short-time centrifugation combined microfluidic detection chip with a three-layer structure, featuring a sample loading area with a slope structure and cylindrical convex drainage points, a double-sided adhesive layer dividing the sample flow channel, and a waste liquid tank area, allowing for capillary-driven sample flow and efficient chromatographic reaction without whole blood filtration, along with a fixing device for stability and a centrifugal detection device for high-speed operation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If whole blood filtration is performed using a filtering membrane, then erythrocytes are removed from the sample, but detection interference occurs and sample is wasted
Solution Approach 1:
The invention removes the filtering membrane component entirely from the microfluidic chip design. Instead of filtering whole blood through a membrane, the system allows whole blood to flow directly through the microfluidic channels to the detection area, eliminating the source of detection interference and sample waste associated with membrane filtration
Solution Approach 2:
The invention introduces magnetic particles as an intermediary carrier that binds to target analytes in whole blood. These magnetic particles facilitate the separation and detection process without requiring membrane filtration, thereby eliminating detection interference while preserving sample integrity
2Volume of moving object
If serpentine tube-like reaction area is used, then the chip structure is compact, but sample remains in the channel which adversely affects detection sensitivity
Solution Approach 1:
The invention transforms the static serpentine channel design into a dynamic system where magnetic particles actively transport the sample through the microfluidic channels. This dynamic transport prevents sample stagnation in the channels while maintaining a compact chip structure, thereby preserving detection sensitivity
Solution Approach 2:
The invention replaces the passive mechanical flow through serpentine channels with an active magnetic field-driven transport system. Magnetic particles respond to external magnetic fields to move samples through the chip, eliminating the need for complex channel geometries and preventing sample retention that reduces sensitivity
3Device complexity
If directly opening channels on the substrate is performed, then the chip structure is simple, but processing cost is high
Solution Approach 1:
The invention employs thin film technology to create the microfluidic chip structure. Thin films are deposited and patterned to form channels and functional areas, providing a balance between structural simplicity and manufacturing cost-effectiveness while enabling precise control over fluid flow paths
4Device complexity
If centrifugal force is used to control liquid flow, then the system is simple, but liquid flow rate is too fast which is not conducive to chromatography reaction progress
Solution Approach 1:
The invention replaces centrifugal force-driven flow with magnetic field-driven transport of magnetic particles. This substitution allows for precise control of sample movement through the microfluidic channels at optimal speeds for chromatography reactions, while maintaining system simplicity through the use of external magnetic fields rather than mechanical centrifugation devices
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This design enhances sample utilization efficiency, reduces non-specific binding, improves detection sensitivity, simplifies chip preparation and assembly, and allows for precise, high-sensitivity immunodetection suitable for clinical point-of-care applications, with reduced equipment requirements and faster analysis times.
Implementation Method 1
a sample flow channel is divided by an adhesive area and an adhesive-free area on the double-sided adhesive layer
Implementation Method 2
the lower layer of the chip is provided with a slope structure or a groove
Implementation Method 3
a centrifugal detection device for stability and a centrifugal detection device for high-speed operation
Data Source
Figure 1~2
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Figure 5~6
AI summary
The disclosure provides a microfluidic detection chip, a preparation method thereof, a fixing device and a centrifugal detection device. The detection chip comprises overlapped three layers. The upper layer of the chip includes a sample loading area and vents; the lower layer of the chip includes a waste liquid tank area in which a slope structure or a groove is disposed; the intermediate layer of the chip is a double-sided adhesive layer on which sample flow channels are divided by an adhesive area and an adhesive-free area. The self-driving and short-time centrifugation combined microfluidic detection chip technology designed by the present disclosure can solve inherent problems of traditional paper substrates, further improve sample utilization, detection speed and detection sensitivity in immunoassay, and the preparation and assembly of the chip are simple, has low requirement for the detection device, and can be conveniently applied to the clinical detection.