Microfluidic DEP and Electrowetting Section Control

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Solution Overview

Problem

Current microfluidic systems face challenges in selectively generating net dielectrophoresis (DEP) forces and changing effective wetting properties of electrowetting surfaces, which limits their ability to efficiently manipulate and move micro-objects and liquid droplets within the devices.

Innovation Solution

The integration of a DEP configuration to induce net DEP forces in a first section of a microfluidic apparatus and an electrowetting (EW) configuration to change the effective wetting properties of an electrowetting surface in a second section, allowing for the capture and movement of micro-objects and liquid droplets across boundaries between different liquid media.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If a DEP configuration is used to generate net DEP forces in a first section, then micro-objects can be captured and moved in the first liquid medium, but the system cannot simultaneously change wetting properties in another section

Engineering Contradiction:
Improvefunctional versatilityVSAvoiddevice complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The device is divided into distinct functional sections: a first section with DEP configuration for micro-object manipulation and a second section with EW configuration for droplet manipulation. Each section is independently controlled and optimized for its specific function, allowing the system to perform multiple operations without requiring a single complex mechanism to handle all tasks.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The microfluidic device integrates multiple functions into a single platform by combining DEP and EW configurations. The device can simultaneously or sequentially perform micro-object capture, droplet manipulation, and fluid transport, making it a multi-functional system that addresses diverse operational requirements without needing separate devices.

Inventive Principle:
Principle #6Universality (Multi-functionality)

2Ease of operation

If electrowetting configuration is added to change wetting properties, then droplet manipulation is enabled, but the system becomes more complex

Engineering Contradiction:
Improvedroplet manipulation capabilityVSAvoiddevice complexity
Core Design Contradiction:
Ease of operationVSDevice complexity

Solution Approach 1:

The electrowetting function is isolated to a specific second section of the device, separate from the DEP section. This segmentation allows droplet manipulation capabilities to be added without interfering with the micro-object manipulation functions in the first section, and enables independent optimization of each functional area.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The device uses electric fields as an intermediary mechanism to control wetting properties through the EW configuration. By applying voltage to the EW section, the effective wetting property of the electrowetting surface is changed, enabling droplet movement and manipulation without mechanical intervention, thus simplifying operation despite the added functional complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Adaptability or versatility

If separate DEP and EW configurations are used in different sections, then both micro-object and droplet manipulation are achieved, but manufacturing becomes more difficult

Engineering Contradiction:
Improvemanipulation capabilityVSAvoidmanufacturing ease
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The DEP and EW configurations are merged into a single integrated microfluidic device structure. Both configurations share common elements such as the microfluidic channels, substrate, and control electronics, allowing the device to manufacture as a unified system rather than separate components that would need to be assembled, thereby reducing overall manufacturing complexity despite the multiple functions.

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach enables precise manipulation and transfer of micro-objects and liquid droplets between different sections of the microfluidic apparatus, enhancing the capability to process and analyze biological samples by combining DEP-induced forces with EW-controlled surface properties.

Implementation Method 1

The DEP configuration is configured to selectively induce net DEP forces in a first liquid medium disposed on the first surface

Methodology Applied
Scientific EffectDielectrophoresis: Dielectric

Implementation Method 2

the EW configuration is configured to selectively change an effective wetting property of the electrowetting surface

Methodology Applied
Scientific EffectElectrowetting: Electrowetting

Implementation Method 3

changing an effective electrowetting characteristic of a region of the electrowetting surface at a boundary with the first surface and thereby induce a force at the boundary that is sufficient to draw a droplet across the boundary

Methodology Applied
Scientific EffectElectrowetting: Electrowetting

Data Source

PatentEP3134738B1DEP force control and electrowetting control in different sections of the same microfluidic apparatus
Publication Date: 2019.07.24 BERKELEY LIGHTS INC
  • EP3134738B1 patent drawingFigure 1A
  • EP3134738B1 patent drawingFigure 1B~1C
  • EP3134738B1 patent drawingFigure 2~3

AI summary

A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing an effective wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.