Microfluidic Electrophoretic Assay for Multiplexed Biomarker Detection

The device segments the confirmatory assay into two independent microfluidic channels: a first channel for detecting first biomarkers and a second channel for detecting second biomarkers. Each channel contains immobilized capture members that specifically bind to target biomarkers, allowing parallel processing of multiple analytes simultaneously. This segmentation enables the assay to be performed in a single laboratory setting without requiring centralized facilities, while maintaining the sensitivity and specificity of conventional blotting assays.

The invention merges multiple assay functions into a single integrated microfluidic device. Both biomarker detection channels are incorporated into one device with shared fluidic components, including a common sample inlet and buffer reservoirs. This merging reduces the overall complexity compared to performing separate blotting assays, eliminates the need for multiple separate laboratory setups, and reduces labor requirements while maintaining detection accuracy through the specific binding of capture members to target biomarkers.

The microfluidic device is designed for self-service operation with automated fluid handling through electrophoretic pumping. The device contains integrated buffer reservoirs that automatically provide washing and blocking buffers during the assay process, eliminating the need for manual intervention at each step. The electrophoretic pumping system automatically moves samples and reagents through the channels based on applied voltage, requiring minimal operator training. This self-service capability enables the device to be operated in resource-constrained settings without requiring specialized laboratory infrastructure or highly trained personnel, while maintaining diagnostic accuracy through the specific capture member-biomarker binding.