Microfluidic Fluorescence Detection Data Segmentation
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Solution Overview
Problem
Current fluorescence detection systems in microfluidic chips face challenges in accurately and efficiently processing large amounts of data from multiple microchambers, leading to communication speed issues and increased processing time, especially during real-time PCR analysis.
Innovation Solution
A fluorescence detection system that includes a chip mount, an optical device for scanning the microfluidic chip, a measuring unit for converting optical signals to digital signals, and a calculator that identifies the microchamber positions and calculates the fluorescence amount by transmitting data as one value per microchamber, reducing data transmission and processing load.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If fluorescence detection is performed on multiple microchambers simultaneously, then analysis throughput is improved, but data transmission and processing time increases
Solution Approach 1:
The patent segments the fluorescence detection process by assigning dedicated detection channels to specific microchambers. Each microchamber is monitored by a specific detection channel, allowing simultaneous detection of multiple samples without creating a single large data processing bottleneck. This segmentation enables parallel processing of fluorescence signals from different microchambers, maintaining high throughput while keeping individual data processing tasks manageable and efficient.
2Measurement precision
If precise fluorescence measurement is performed, then measurement accuracy is improved, but communication speed between detection device and calculator deteriorates
Solution Approach 1:
The patent divides the fluorescence detection system into multiple independent detection channels, each handling a specific microchamber. This segmentation allows precise fluorescence measurements to be performed in parallel across multiple channels without overwhelming the communication system. Each channel transmits data independently at a manageable rate, maintaining both measurement precision and communication speed by avoiding a single large data transmission bottleneck.
Solution Approach 2:
The patent introduces a data processing unit that acts as an intermediary between the optical detection device and the calculator. This intermediary unit receives fluorescence signals from multiple detection channels, performs preliminary processing and aggregation of the data, and then transmits the processed results to the calculator. This intermediate processing step reduces the communication load on the main calculator while maintaining precise measurement capabilities, as the intermediary can handle data aggregation efficiently before final analysis.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces data transmission and processing time, enabling efficient analysis of multiple microchambers by transmitting data as one value per microchamber, thereby enhancing communication speed and calculation efficiency.
Implementation Method 1
an optical device for emitting light on the microfluidic chip and detecting fluorescence which is generated from a fluorescent material existing in the microfluidic chip
Data Source
AI summary
A system and a method for detecting fluorescence in a microfluidic chip having a microchamber and measuring the amount of the fluorescence are disclosed. In the system and method, an optical device detects fluorescence generated by a fluorescent material. The position of the outer circumference of a microfluidic chip is identified based on the change of the amount of the detected fluorescence. Then, the position of a microchannel is calculated based on the position of the outer circumference of the microfluidic chip and the distance between the outer circumference and the microchamber. Hence, the amount of the fluorescence only generated from the microchamber of the microfluidic chip is automatically calculated. Further, a plurality of samples can be analyzed at the same time, thereby reducing analysis time and cost.