Microfluidic Particle Positioning via Intermittent Flow Control

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Solution Overview

Problem

Existing microfluidic systems for analyzing particles, particularly biological materials, face challenges in precisely controlling the position of particles in three-dimensional space without subjecting them to mechanical, chemical, or biological stresses, and often require complex and costly setups that are not suitable for flexible repositioning or efficient fluid use.

Innovation Solution

A device and method that utilize a detection and control unit to generate temporary positioning flows in a positioning fluid, allowing direct control of particle movement in any spatial direction within the analysis chamber, avoiding the need for traps and minimizing fluid usage by using intermittent, one-way flows.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If optical traps, dielectrophoretic traps, magnetic traps, or mechanical traps are used to control particle position, then positioning precision is improved, but device complexity and cost increase

Engineering Contradiction:
Improvepositioning precisionVSAvoiddevice complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent replaces complex optical, electromagnetic, and mechanical trapping systems with a simple microfluidic flow-based positioning system. By using controlled fluid flows generated through microchannels to transport particles to desired locations, the system eliminates the need for lasers, electromagnetic fields, or mechanical traps, thereby reducing device complexity and cost while maintaining positioning capability

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent employs hydraulic principles by using controlled fluid flows through microchannels to position particles. The system utilizes pump-driven or pressure-controlled fluid delivery to create定向 flows that transport particles to specific locations within the analysis chamber, providing a simple and effective positioning mechanism

Inventive Principle:
Principle #29Pneumatics and hydraulics

2Measurement precision

If optical traps, dielectrophoretic traps, or mechanical traps are used to control particle position, then positioning precision is improved, but harmful factors affecting the particle increase

Engineering Contradiction:
Improvepositioning precisionVSAvoidmechanical, chemical, and thermal stresses
Core Design Contradiction:
Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

The patent replaces mechanical, optical, and electromagnetic trapping methods that exert forces and stresses on particles with a gentle fluid flow-based positioning system. The microfluidic flows transport particles using viscous drag forces that are sufficiently gentle to avoid damaging biological samples, thereby eliminating harmful mechanical, thermal, and chemical stresses while maintaining positioning precision

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Measurement precision

If fluid-dynamic traps with continuous flows are used to control particle position, then positioning is achieved, but fluid consumption increases

Engineering Contradiction:
Improvepositioning capabilityVSAvoidfluid consumption
Core Design Contradiction:
Measurement precisionVSLoss of substance

Solution Approach 1:

The patent implements periodic or intermittent fluid flows instead of continuous flows for particle positioning. The system activates flows only when particle repositioning is required and deactivates them when the particle is at the desired location, thereby reducing overall fluid consumption while maintaining effective positioning capability

Inventive Principle:
Principle #19Periodic action

Solution Approach 2:

The patent employs dynamic flow control where the magnitude and direction of fluid flows are adjusted based on real-time particle position feedback. The system uses variable flow rates through different microchannels to efficiently transport particles to target locations without maintaining constant high-flow conditions, thereby optimizing fluid usage

Inventive Principle:
Principle #15Dynamics

4Measurement precision

If fluid-dynamic traps with lateral channels are used to control particle position, then trapping is achieved, but repositioning flexibility is reduced

Engineering Contradiction:
Improvetrapping capabilityVSAvoidrepositioning flexibility
Core Design Contradiction:
Measurement precisionVSAdaptability or versatility

Solution Approach 1:

The patent divides the positioning system into multiple independent microchannels that can be individually controlled. Each microchannel can be activated or deactivated independently to transport particles to different locations within the analysis chamber, providing flexible repositioning capability while maintaining effective trapping when needed

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs dynamic flow control where the magnitude and direction of fluid flows are adjusted based on real-time particle position feedback. The system uses variable flow rates through different microchannels to efficiently transport particles to target locations, enabling flexible repositioning to any location within the chamber

Inventive Principle:
Principle #15Dynamics

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables precise, stress-free control of particle position in three-dimensional space, reducing the complexity and cost of the analysis process while maintaining particles at predefined positions, such as the focal point of optical devices, and allowing for flexible movement and positioning without continuous fluid flows.

Implementation Method 1

a positioning unit adapted, during a particle analysis operation, to be activated and deactivated on the basis of the detected parameter of the particle, to generate a temporary positioning flow in the positioning fluid, which drives or controls the position of the particle so as to move it into a predefined position in the analysis chamber

Methodology Applied
Scientific EffectFluid flow:

Data Source

PatentEP3233283B1Device for real time analysis of particles suspended in a fluid and method for the analysis of said particles
Publication Date: 2021.06.16 CELLDYNAMICS I SRL
  • EP3233283B1 patent drawingFigure 1~2
  • EP3233283B1 patent drawingFigure 3~4
  • EP3233283B1 patent drawingFigure 5~6

AI summary

The present invention refers to a device for the analysis of a particle comprising an analysis chamber adapted to contain a positioning fluid. A parameter of the particle suspended in the positioning fluid is detected by means of a detection and control unit. A positioning unit, during a particle analysis operation, is activated and deactivated on the basis of the detected parameter of the particle. The detection and control unit can activate the at least one positioning unit so as to generate a temporary positioning flow in the positioning fluid, such that said temporary positioning flow acts directly on the particle and drives the position of the particle so as to move it into a predefined position in the analysis chamber. The detection and control unit can also deactivate the at least one positioning unit when the particle to be analyzed is in the predefined position, such that the positioning fluid is at rest.