Microfluidic Sampling and Sealing Structure for Automated Mixing

The patent combines the sampling structure and mixing structure into a single integrated microfluidic chip. The sampling structure includes a sampling channel with partition columns that directly connect to the mixing chamber, eliminating the need for external sampling operations. The mixing structure includes a mixing channel with rotating columns that integrate mixing functionality within the chip itself, achieving both sampling and mixing automation without requiring external devices.

The microfluidic chip is designed as a universal platform that performs multiple functions: sampling through the sampling channel, mixing through the rotating mixing columns, and detection. The partition columns and rotating mixing columns serve dual purposes of fluid guidance and active mixing, making the device multi-functional while maintaining a compact integrated structure.

The mixing structure employs rotating mixing columns that can rotate around a central axis, introducing dynamic motion to the mixing process. This rotational movement creates enhanced fluid circulation and mixing compared to static mixing structures, significantly improving mixing efficiency and uniformity while the rotation mechanism remains integrated within the chip.

The rotating mixing columns generate mechanical motion and fluid circulation patterns that enhance mixing. The rotation creates vortex flows and repeated fluid redistribution, achieving rapid and uniform mixing of samples and reagents within the mixing chamber, thereby improving productivity without requiring complex external mixing equipment.

The exhaust hole is designed with an elastic sealing component that can dynamically change its state. The elastic component can be deformed by external pressure to open or close the exhaust hole, allowing the sealed structure to adapt its permeability based on operational needs. This dynamic control enables reliable sealing during operation while allowing pressure equalization and exhaust when required.

The sealing structure uses an elastic component whose physical state (open/closed) can be changed by applying different pressure parameters. By controlling the pressure applied to the elastic sealing element, the system can switch between sealed and vented states, maintaining reliability through sealing while enabling ease of operation through pressure-controlled exhaust functionality.