Purified Micronized Fat Cells for Joint and Muscle Repair

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Solution Overview

Problem

Current treatments for osteoarthritis and muscle damage, such as hyaluronic acid injections and fat cell transplants, face issues with durability, necrosis, and impurities leading to poor efficacy and potential complications like scarring and immunorejection.

Innovation Solution

A cell preparation comprising micronized fat cells and adipose stem cells, obtained by separating and purifying adipose tissue, which are then micronized and concentrated to ensure high stem cell density, enhancing tissue regeneration and reducing pain in osteoarthritis and muscle damage.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of operation

If hyaluronic acid is injected to increase soft tissue volume, then the treatment is easy to administer, but the injected hyaluronic acid is absorbed in about half a year resulting in poor durability

Engineering Contradiction:

Improveease of administrationVSAvoiddurability of effect

Core Design Contradiction:

Ease of operationVSDuration of action of stationary object

Solution Approach 1:

The patent changes the fundamental parameter from using synthetic hyaluronic acid to using living adipose stem cells, which have the capability to self-renew and differentiate, thereby providing long-term regenerative effects rather than temporary filling

Inventive Principle:

Principle #35Parameter changes

2Duration of action of stationary object

If fat cells are transplanted to increase soft tissue volume, then the effect can be durable, but the fat cells sometimes necrose or are absorbed after transplantation requiring reoperation or causing scars

Engineering Contradiction:

Improvedurability of effectVSAvoidsuccess rate of transplantation

Core Design Contradiction:

Duration of action of stationary objectVSReliability

Solution Approach 1:

The patent extracts and removes impurities (blood, fatal/vital cells, senescent cells) from the adipose tissue mixture through centrifugation and filtration, isolating only the healthy adipose stem cells and fat cells that have high survival rates after transplantation

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent changes from using whole fat tissue or crude fat cell suspensions to using purified adipose stem cells, fundamentally altering the cellular composition to improve engraftment and reduce necrosis

Inventive Principle:

Principle #35Parameter changes

3Reliability

If adipose stem cells are used to improve fixation ratio and regeneration, then soft tissue regeneration is enhanced, but the obtained cells may include impurities causing necrosis or calcification

Engineering Contradiction:

Improvefixation ratio of cellVSAvoidimpurities causing necrosis

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent systematically removes impurities including blood cells, fatal/vital cells, and senescent cells from the adipose tissue through centrifugation and filtration processes, extracting only the beneficial adipose stem cells

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent applies different processing conditions to different components of the adipose tissue: gentle centrifugation to preserve stem cells, filtration to remove impurities, and careful handling to maintain cell viability, creating a locally optimized preparation process

Inventive Principle:

Principle #3Local quality

4Quantity of substance

If a mixture including fat cells and adipose stem cells is obtained by liposuction, then both cell types are collected, but the mixture includes a wide variety of impurities requiring removal

Engineering Contradiction:

Improveconcentration of useful cellsVSAvoidimpurities in mixture

Core Design Contradiction:

Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The patent extracts useful cells (adipose stem cells and fat cells) from the complex liposuction mixture by centrifugation and filtration, separating them from blood, tissue debris, and other impurities

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent segments the liposuction mixture into different components through centrifugation: the upper layer containing oil and debris is discarded, the middle layer containing adipose stem cells is collected, and the lower layer containing blood is discarded, achieving separation of useful cells from harmful impurities

Inventive Principle:

Principle #1Segmentation

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The cell preparation effectively alleviates pain, promotes tissue regeneration in joints and muscles, and is safer due to autologous origin and absence of cell culture steps, reducing infectious disease risks and immunorejection.

Implementation Method 1

separating an adipose tissue taken from a subject into a cellular fraction and a liquid fraction to obtain the cellular fraction

Methodology Applied

Scientific EffectCentrifugal separation: Centrifugal Separation

Implementation Method 2

removing impurities from the cellular fraction to obtain a concentrate of a fat cell and an adipose stem cell

Methodology Applied

Scientific EffectFiltration: Filter (physical)

Data Source

PatentEP2818173B1Cell preparation including fat cell

Publication Date: 2021.09.22 SAEKI MASANORI

Figure 1(a)~2(b)
Figure 3

AI summary

[Problem] A main objective of the present invention is to provide a cell preparation useful for treatment of diseases in bone joints and repairing muscles which have been degenerated or damaged. [Solution] The present invention provides a cell preparation for treatment of diseases in bone joints which comprises a fat cell, and a cell preparation for repairing muscles which comprises a fat cell and a mesodermal stem cell.