Microparticle Analyzer Multi-Wavelength Fluorescence Spectrum Display
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing flow cytometers face challenges in accurately analyzing multicolor fluorescence data due to spectral overlap from multiple fluorescent dyes, leading to reduced analysis precision and difficulty in extracting spectrum information.
Innovation Solution
A microparticle analyzing apparatus and method that simultaneously detects fluorescence in multiple wavelength regions, allowing for the display of detection results as spectra, enabling users to visually understand the data with or without fluorescence correction and extracting specific information from the microparticles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple fluorescent dyes are used in multicolor analysis, then the analysis capability is enhanced, but spectral overlap occurs reducing measurement precision
Solution Approach 1:
The detection system is segmented into multiple wavelength regions with separate sensors, allowing independent detection of fluorescence signals across different spectral ranges. This segmentation enables the system to handle multiple fluorescent dyes by assigning specific wavelength regions to specific dyes, reducing spectral overlap interference while maintaining comprehensive multicolor analysis capability.
2Measurement precision
If fluorescence correction is applied to extract objective fluorescent dye information, then measurement precision is improved, but spectrum information is lost
Solution Approach 1:
The system transitions from traditional single-wavelength detection to multi-wavelength spectral detection by adding a wavelength dimension. Multiple sensors detect fluorescence across different wavelength regions simultaneously, creating a spectral profile that preserves both the intensity information needed for precision measurement and the wavelength distribution information needed for spectrum analysis. This dimensional expansion allows simultaneous achievement of measurement precision and spectrum information retention.
3Ease of operation
If traditional flow cytometry detects light at specific bandwidth, then the detection process is simplified, but spectrum information cannot be extracted
Solution Approach 1:
The detection system is designed with multi-functionality, where multiple sensors equipped with different wavelength region sensitivities can simultaneously perform both simplified fluorescence detection and spectrum information extraction. Each sensor maintains ease of operation for its designated wavelength range while collectively providing comprehensive spectral data, allowing the system to serve both simplified detection needs and sophisticated spectrum analysis requirements without compromising either function.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables precise analysis and visualization of fluorescence spectra from microparticles, improving data interpretation and obtaining various pieces of information without the need for fluorescence correction, and facilitating more accurate multicolor analysis.
Implementation Method 1
a detecting portion that simultaneously detects a fluorescence generated from a microparticle in plural wavelength regions
Data Source
AI summary
Disclosed herein is a microparticle analyzing apparatus including a detecting portion configured to simultaneously detect a fluorescence generated from a microparticle in plural wavelength regions and a displaying portion configured to display thereon detection results in the plural wavelength regions in a form of a spectrum.