Microparticle Reagent Multiplexed SARS-CoV-2 Antigen Antibody Detection
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Solution Overview
Problem
There is a need for effective methods to assess the presence and quantity of SARS-CoV-2 antigens and antibodies in biological samples, particularly for rapid and accurate diagnosis and monitoring of COVID-19 infections.
Innovation Solution
A method involving microparticle reagents that specifically bind to SARS-CoV-2 antigens and antibodies, with detectable labels forming complexes to indicate the presence or amount of these components in biological samples, allowing for simultaneous or sequential detection in a biological sample.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If conventional separate detection methods are used for SARS-CoV-2 antigens and antibodies, then detection accuracy is maintained, but detection time and procedural complexity increase
Solution Approach 1:
The patent combines separate antigen and antibody detection into a single multiplexed assay using microparticle-based flow cytometry. Multiple detection targets are simultaneously analyzed in one test, eliminating the need for sequential separate tests and reducing overall detection time while maintaining accuracy.
Solution Approach 2:
The microparticle flow cytometry platform serves multiple detection functions simultaneously - detecting both SARS-CoV-2 antigens and antibodies, as well as other parameters, within a single universal system. This multi-functional approach reduces procedural complexity compared to using separate specialized tests.
2Speed
If rapid detection methods are implemented for SARS-CoV-2, then detection speed increases, but measurement precision may deteriorate
Solution Approach 1:
The patent replaces traditional mechanical/chemical assay methods with flow cytometry detection, which uses optical scattering and fluorescence detection. This substitution enables rapid automated analysis with high precision quantification, achieving both fast detection speeds and accurate measurement through optical physics rather than mechanical processes.
Solution Approach 2:
The use of microparticles with detectable labels creates optical copies or signals that represent the presence and quantity of target antigens and antibodies. These optical signals can be rapidly detected and quantified with high precision, maintaining measurement accuracy while enabling fast throughput.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate detection of SARS-CoV-2 antigens and antibodies in biological samples, facilitating timely diagnosis and monitoring of COVID-19 infections, with the potential for point-of-care applications.
Implementation Method 1
microparticle reagents that specifically bind to SARS-CoV-2 antigens and antibodies
Implementation Method 2
detectable labels forming complexes to indicate the presence or amount of these components
Data Source
AI summary
Disclosed herein are methods, kits, and systems for detecting at least one type of SARS-CoV-2 antigen and at least one type of anti-SARS-CoV-2 antibody in a subject, which comprises the use of at least two different types of microparticle reagents for binding at least one type of SARS-CoV-2 antigen and at least one type of anti-SARS-CoV-2 antibody and at least two different types of detection reagents for binding each of the microparticle reagents.