Micropore Isothermal Biomolecule Analysis With Low-Adsorption Sealing
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Solution Overview
Problem
Existing biomolecule analysis methods are inefficient, requiring complex procedures, large devices, and prolonged reaction times, especially for single nucleotide polymorphism (SNP) analysis, due to the need for PCR amplification and isothermal reactions that lack reactivity and accuracy.
Innovation Solution
A biomolecule analysis kit and method utilizing a reaction container with low-adsorption structural portions and surfactants to minimize sample and reagent adsorption, enabling rapid and quantitative analysis through isothermal enzymatic reactions in microspaces.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If PCR amplification is used for SNP analysis, then the target gene can be amplified rapidly, but the device size increases and heat-resistant reaction containers are required
Solution Approach 1:
The patent changes the temperature parameter from variable (PCR) to constant (isothermal), enabling the reaction to proceed at a single temperature (e.g., 37°C) without thermal cycling. This eliminates the need for complex heating/cooling mechanisms and heat-resistant containers, while maintaining high amplification efficiency through the specific isothermal nucleic acid amplification reaction conditions.
2Device complexity
If conventional reaction containers are used, then the structure is simple, but sample and reagent adsorption increases leading to reduced reactivity and higher background noise
Solution Approach 1:
The patent applies low-adsorption treatment specifically to the inner surface of the reaction container where the sample and reagents contact, while the rest of the container maintains its simple structure. This localized modification reduces non-specific adsorption of biomolecules and reagents at the critical reaction interface, improving reaction efficiency and signal-to-noise ratio without complicating the overall device design.
3Ease of operation
If isothermal reactions are performed without low-adsorption treatment, then the procedure is simple, but the reaction time is prolonged and accuracy is reduced
Solution Approach 1:
The patent optimizes the isothermal reaction temperature and time parameters to achieve rapid amplification. By conducting the reaction at a specifically controlled isothermal condition (e.g., 37°C for a defined period), the patent achieves both simplicity of operation and rapid signal detection, eliminating the need for complex thermal cycling while maintaining short reaction times through optimized isothermal conditions.
Solution Approach 2:
The patent replaces the mechanical thermal cycling system with a simple isothermal heating system, substituting complex temperature cycling mechanics with a constant temperature maintenance system. This substitution simplifies the operational procedure while achieving rapid and accurate results through the efficiency of the isothermal nucleic acid amplification reaction.
4Ease of manufacture
If standard reaction containers are used, then manufacturing is simple, but background noise increases reducing measurement precision
Solution Approach 1:
The patent applies low-adsorption treatment only to the inner surface of the reaction container that contacts the sample and reagents, while the outer structure and other components remain simple to manufacture. This localized treatment reduces background noise from non-specific adsorption at the reaction interface, improving SNP detection accuracy without significantly complicating the overall manufacturing process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution allows for rapid and accurate biomolecule analysis with improved reactivity and reduced background noise, enabling efficient detection of genetic variations like SNPs in a shorter timeframe.
Implementation Method 1
a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion
Implementation Method 2
the reagent contains a surfactant for reducing the surface tension of the reagent
Implementation Method 3
a reaction container configured to perform an enzymatic reaction
Data Source
AI summary
A biomolecule analysis method may include preparing a reaction container comprising an array of a plurality of micropores in which a wash buffer is filled or preparing the reaction container and feeding the wash buffer into the reaction container including the array of the plurality of micropores. The method may include feeding a reaction reagent to detect a sample, into the plurality of micropores, the sample including a biomolecule. The method may include feeding a sealing solution to seal the plurality of micropores so that the plurality of micropores become a plurality of independent reaction chambers sealed from each other. The method may include causing an isothermal detection reaction in each of the plurality of the independent reaction containers. The method may include detecting a signal amplified by the isothermal detection reaction.