Micropore Reaction Chamber Sealing for Rapid Biomolecule Detection
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Solution Overview
Problem
Existing biomolecule analysis methods, such as PCR and INVADER, are either complex and require multiple stages or take too long to achieve accurate SNP detection, lacking efficiency and practicality.
Innovation Solution
A biomolecule analysis kit and method utilizing a reaction container with a low-adsorption structural portion and surfactant-containing reagents to perform enzymatic reactions, allowing for rapid and quantitative analysis by minimizing adsorption and optimizing signal detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If PCR method is used for gene amplification, then amplification efficiency is improved, but device complexity and operation complexity increase due to temperature control requirements
Solution Approach 1:
The patent replaces the mechanical thermal cycling system (heating/cooling devices) with a chemical isothermal amplification system. The Bst DNA polymerase enables DNA amplification at a constant temperature (65°C), eliminating the need for complex temperature programming hardware and software control systems while maintaining high amplification efficiency.
Solution Approach 2:
The patent changes the operational temperature parameter from variable (PCR cycling between 94°C, 55°C, 72°C) to constant (65°C isothermal). This parameter change simplifies the device requirements while preserving amplification capability, as the Bst polymerase is specifically engineered to function optimally at this constant temperature.
2Measurement precision
If two-stage operation (amplification + detection) is performed, then detection accuracy is improved, but analysis time increases
Solution Approach 1:
The patent merges the amplification and detection stages into a single integrated isothermal reaction process. The amplification primers are designed with detection functionality, allowing simultaneous amplification and SNP detection at 65°C without requiring separate thermal cycling and detection equipment, thereby reducing total analysis time while maintaining accuracy.
Solution Approach 2:
The patent creates a multi-functional reagent system where the same isothermal amplification reaction serves both amplification and detection purposes. The assay design allows the reaction to simultaneously generate amplified DNA products and produce detectable signals for SNP identification, eliminating the need for separate specialized stages.
3Ease of manufacture
If conventional reaction containers are used, then manufacturing simplicity is maintained, but adsorption of biomolecules increases reducing signal intensity
Solution Approach 1:
The patent applies low-adsorption coating only to the inner surface of the reaction container where the biomolecular reaction occurs. This localized treatment prevents adsorption of DNA, enzymes, and other reactants at the critical reaction interface while keeping the rest of the container simple and cost-effective to manufacture.
Solution Approach 2:
The patent uses composite material structure for the reaction container, combining a conventional container body with a specialized low-adsorption coating layer on the inner surface. This composite approach maintains manufacturing simplicity for the bulk material while adding functional properties where needed to prevent biomolecule adsorption and preserve signal intensity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables rapid and quantitative biomolecule analysis with improved reactivity, reducing detection time and enhancing signal intensity and accuracy.
Implementation Method 1
a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion
Implementation Method 2
the reagent contains a surfactant
Data Source
AI summary
A biomolecule analysis method may include preparing a reaction container comprising an array of a plurality of micropores in which a wash buffer is filled or preparing the reaction container and feeding the wash buffer into the reaction container including the array of the plurality of micropores. The method may include feeding a reaction reagent to detect a sample, into the plurality of micropores, the sample including a biomolecule. The method may include feeding a sealing solution to seal the plurality of micropores so that the plurality of micropores become a plurality of independent reaction chambers sealed from each other. The method may include causing an isothermal detection reaction in each of the plurality of the independent reaction containers. The method may include detecting a signal amplified by the isothermal detection reaction.