MicroRNA Markers for Identifying Anti-Aging Extracts
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Solution Overview
Problem
Current anti-aging skin care products lack effective methods to address endogenous skin aging, which is influenced by genetic changes and external factors, and there is a need for innovative approaches to improve skin aging using microRNA markers.
Innovation Solution
The use of hsa-miR-30c-2-3p and hsa-miR-125b-1-3p as microRNA markers to determine the effectiveness of snow ginseng extract in improving aged skin cells, where these microRNAs target the IGF1 and IGFIR genes involved in skin aging pathways.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If conventional skin care products are used to address skin aging, then external factors such as ultraviolet irradiation and environmental pollution can be mitigated, but endogenous aging caused by genetic changes cannot be effectively addressed
Solution Approach 1:
The patent introduces microRNA markers (hsa-miR-30c-2-3p and hsa-miR-125b-1-3p) as intermediaries to bridge the gap between conventional skin care products and endogenous aging. These microRNA markers serve as detection tools that can identify the effectiveness of extracts in addressing endogenous aging, enabling a new approach to validating anti-aging product efficacy that goes beyond traditional external factor protection
Solution Approach 2:
The patent replaces traditional mechanical or chemical detection methods with a molecular biology-based detection system using microRNA markers. Instead of relying on conventional assays to measure anti-aging effects, the invention uses RNA interference mechanisms and microRNA expression analysis to detect and validate the impact of extracts on endogenous aging pathways, providing a more precise and reliable measurement approach
2Measurement precision
If microRNA markers are used to detect the effectiveness of extracts, then the precision of effectiveness determination is improved, but the complexity of the detection system increases
Solution Approach 1:
The patent extracts and focuses on specific microRNA markers (hsa-miR-30c-2-3p and hsa-miR-125b-1-3p) from the complex microRNA landscape. By isolating these two key markers that are specifically down-regulated by effective extracts, the invention simplifies the detection system while maintaining high precision. This selective extraction of critical markers allows for targeted detection without requiring comprehensive analysis of all microRNAs
Solution Approach 2:
The patent uses RT-PCR technology to create complementary DNA copies of the microRNA markers, enabling their detection and quantification. This copying mechanism allows for sensitive and specific detection of the microRNA markers in skin cell extracts, providing a reliable method to assess extract effectiveness without directly analyzing the complex RNA structures
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The snow ginseng extract composition effectively down-regulates the microRNA markers hsa-miR-30c-2-3p and hsa-miR-125b-1-3p in aged skin cells, indicating its potential to improve endogenous skin aging by regulating key genes involved in skin regeneration and longevity.
Implementation Method 1
The microRNA is capable of targeting messenger RNA (mRNA), and causing the mRNA degradation thereof or terminating the mRNA translation thereof. Such microRNAs thus play a very important regulation role in the cells.
Data Source
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AI summary
The present invention provides a method for screening an endogenous skin ageing target, an active substance for improving endogenous skin ageing and a screening method therefor. In the method, microRNAs in skin cells are used as a test basis, and the microRNA marker is one of hsa-miR-30c-2-3p and hsa-miR-125b-1-3p or a combination thereof. The microRNA marker is capable of inhibiting the expressions of IGF1 gene and IGFIR gene, and thus affecting endogenous skin ageing. The present invention provides a new regulation target for developing novel active ingredients of cosmetics for improving endogenous skin ageing.