Microscopic Imaging System for Sample Angle Recognition
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current microscopic imaging technologies face challenges in accurately characterizing the angular distribution of metal nanomaterials, particularly with traditional optical microscopes, due to limitations in resolution and the complexity of existing polarization imaging methods, which are often expensive and difficult to analyze.
Innovation Solution
A microscopic imaging system and method that utilizes a polarization rotation device to modulate excitation light within a preset angle range, splitting it into two parts for imaging and polarization detection, allowing for the calculation of angle information by comparing sample and model characteristic curves to determine the polarization angle of samples with high accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional optical microscopy or differential interference contrast microscopy is used to image metal nanorods, then the imaging can be performed with simple equipment, but the angular distribution of metal nanorods cannot be accurately characterized
Solution Approach 1:
The excitation light beam is divided into two separate beams: a first excitation light for imaging and a second excitation light for polarization detection. This segmentation allows independent optimization of imaging quality and polarization measurement, resolving the contradiction between measurement precision and device complexity.
Solution Approach 2:
The system uses a single light source that serves dual purposes: providing excitation light for imaging metal nanorods and providing reference light for polarization angle detection. This multi-functionality reduces device complexity while maintaining high angular characterization precision.
2Measurement precision
If electron microscopy (scanning electron microscopy, transmission electron microscopy) is used to characterize metal nanorod morphology, then extremely high spatial resolution is achieved, but the method is difficult to apply to dynamic study of biological samples
Solution Approach 1:
The patent replaces electron microscopy (which uses electron beams and vacuum environments) with optical microscopy using polarized light. This substitution maintains sufficient measurement precision for angular characterization while enabling dynamic study of living biological samples in physiological conditions.
3Measurement precision
If existing polarization imaging techniques are combined with traditional optical microscopy to achieve angle resolution, then the translation and rotation diffusion of metal nanorods can be realized, but the optical system becomes complex and data analysis becomes difficult
Solution Approach 1:
The patent segments the excitation light into two distinct beams with specific functions: one for imaging and one for polarization reference. This simplifies the optical system architecture compared to traditional polarization imaging while maintaining angle resolution capability.
Solution Approach 2:
The second excitation light serves as an intermediary reference beam that carries polarization angle information without directly interacting with the sample. This intermediary approach simplifies data analysis by providing a direct reference for polarization state without complex sample-light interaction dynamics.
4Measurement precision
If existing polarization imaging schemes are used, then angle information can be obtained, but the equipment becomes expensive and imaging data analysis becomes difficult
Solution Approach 1:
The system achieves polarization angle detection using standard optical components (beam splitter, polarizers, detectors) that are commonly available in optical microscopy setups. This multi-functional approach uses existing equipment for both imaging and polarization measurement, reducing equipment cost while maintaining detection precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables simple and cost-effective angle recognition of samples, improving the characterization of metal nanorods and other polarized light-responsive materials, enhancing the study of biological systems and materials science with high spatial and temporal resolution.
Implementation Method 1
a polarization rotation device for modulating a polarization angle of the excitation light to obtain a polarization-modulated excitation light
Implementation Method 2
a beam splitting device for splitting the polarization-modulated excitation light into two parts including a first excitation light and a second excitation light
Implementation Method 3
an imaging device for detecting an intensity of fluorescence or reflected light emitted by the object to be analyzed in the sample after being excited by the first excitation light
Implementation Method 4
a polarization detection device for detecting change information about a polarization angle of the second excitation light to obtain polarization change information of the polarization-modulated excitation light
Data Source
AI summary
Discloses are a microscopic imaging system and a microscopic imaging method for sample angle recognition. Firstly, a polarization rotation device introduced in an optical path of an excitation light can polarize and modulate the excitation light so as to make a polarization-modulated excitation light to rotate in a preset angle range; secondly, the polarization-modulated excitation light can be splitted into two parts via a beam splitting device, one for sample imaging, and the other for detection of polarization; and finally, a plurality of model characteristic curves can be obtained by a polarization detection device, each of the model characteristic curves corresponding to one angle, thus determining an angle information about an object to be analyzed in the sample.