Microscope Cell Staining Observation System
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Solution Overview
Problem
Current microscope systems face challenges in efficiently capturing high-resolution images of specimens stained with multiple dyes, particularly in pathological diagnosis, where simultaneous morphological and molecular target observations are difficult due to limitations in dye preservation and the need for specialized equipment.
Innovation Solution
A microscope system that includes an image acquisition unit for capturing images stained with element identification and molecule target dyes, a dye amount calculation unit, an element area identification unit, an extraction condition setting unit, a target portion extraction unit, and a display processing unit to generate and display images of specific cell constituents and target molecules, enabling precise identification and visualization of cellular components and molecular targets.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If fluorescent labeling is used for molecule target staining, then high sensitivity and sharp images can be acquired, but the specimen cannot be preserved for a long period of time and requires a dedicated dark room
Solution Approach 1:
The patent segments the observation process into two distinct modes: fluorescent observation mode for high-sensitivity molecule target detection and bright field observation mode for morphological observation and long-term preservation. This allows the system to switch between different observation methods depending on the diagnostic needs, resolving the contradiction between image quality and specimen preservation duration
Solution Approach 2:
The microscope system is designed with multi-functionality to perform both fluorescent observation and bright field observation using the same specimen. The observation unit can switch between different observation modes, enabling the system to achieve high sensitivity when needed while also allowing long-term preservation and morphological observation, thus eliminating the need for separate specimens or equipment
2Adaptability or versatility
If fluorescent labeling is used for molecule target staining, then a plurality of target molecules can be labeled at the same time, but the observation cannot be performed easily due to long diagnosis time and need for dark room
Solution Approach 1:
The microscope system integrates multiple observation functions into a single platform. The observation unit can perform both fluorescent observation for multi-target molecule labeling and bright field observation for morphological examination, allowing pathologists to conduct comprehensive diagnostics without switching between different equipment or locations, thereby improving ease of operation while maintaining versatility
Solution Approach 2:
The patent merges fluorescent observation capability and bright field observation capability into a single microscope system. This combination allows simultaneous or sequential performance of both molecule target staining observation and morphological observation, eliminating the need for separate dark rooms and equipment, thus improving operational convenience while preserving multi-target labeling capability
3Measurement precision
If fluorescent labeling is used for molecule target staining, then high sensitivity observation is achieved, but it cannot be performed at the same time as morphological observation
Solution Approach 1:
The observation unit is designed with multi-functionality to perform both fluorescent observation and bright field observation. This allows the system to achieve high sensitivity molecule target detection when needed, while also enabling morphological observation, and permits switching between these modes or performing both on the same specimen without requiring separate equipment or specimens
Data Source
AI summary
A microscope system includes an acquisition unit that obtains a specimen image acquired by capturing the specimen stained by an element identification dye that visualizes a predetermined cell constituent element and a molecule target dye that visualizes a predetermined target molecule by using a microscope; a dye amount unit that obtains dye amounts of the element identification dye and the molecule target dye that stain corresponding positions on the specimen for each pixel of the image; an element area identification unit that identifies an area of the cell constituent element based on the dye amount of the element identification dye; a condition setting unit that sets the presence or absence of the predetermined target molecule on the cell constituent element as a condition; and an extraction unit that extracts an area of a target portion that satisfies the condition based on the dye amount of the molecule target dye.