Microscope Correction Collar Dynamic Spherical Aberration Control
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Solution Overview
Problem
In time-lapse microscopy, pre-calculated correction collar settings for spherical aberrations become insufficient due to changes in the sample or microscope apparatus over time, leading to inadequate correction of spherical aberrations during extended observations.
Innovation Solution
A microscope system with a controller that detects a reference target position and updates correction information based on variation information, continuously adjusting the correction collar settings for each position of the objective during the observation period to maintain accurate spherical aberration correction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If pre-calculated correction collar settings are used for time-lapse microscopy, then the observation can proceed efficiently without continuous adjustments, but the spherical aberration correction becomes insufficient due to sample or apparatus changes over time
Solution Approach 1:
The system automatically detects the reference target position during interval periods and uses this feedback to update correction information, creating a closed-loop control system that maintains correction accuracy throughout time-lapse observations without manual intervention
Solution Approach 2:
The correction collar settings are made dynamic by automatically updating correction information based on detected position variations, allowing the system to adapt to changes in sample or apparatus conditions during the observation period
2Reliability
If correction information is updated frequently during time-lapse observation, then spherical aberration correction accuracy is maintained, but the complexity of the control system increases
Solution Approach 1:
The system updates correction information periodically during interval periods between observations, balancing the need for accuracy with system simplicity by using automated detection at scheduled intervals rather than continuous complex control
3Manufacturing precision
If the correction collar is continuously adjusted during observation, then image quality is maintained, but the observation time is extended due to adjustment intervals
Solution Approach 1:
The system performs preliminary detection of reference target position during interval periods before the next observation, allowing correction information to be updated in advance so that the actual observation can proceed without time-consuming adjustments
Data Source
AI summary
A microscope system includes a microscope apparatus and a controller. The microscope apparatus includes an objective and a correction device that corrects a spherical aberration. The controller, during an interval period of a time-lapse observation, detects a reference target position of an observation target and updates correction information on the basis of at least variation information, the correction information representing a relationship between a position of the objective and a setting of the correction device and the variation information representing a variation amount of a distance from the objective to the reference target position. The controller, during an observation period of the time-lapse observation, controls the correction device in accordance with the correction information for each position of the objective determined on the basis of at least the variation information.