Microscope Display Segmentation for Super-Resolution Preview
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Solution Overview
Problem
Current microscope systems face challenges in obtaining desired super-resolution images due to difficulties in determining the appropriate viewing field and imaging parameters, leading to inefficiencies and repeated imaging attempts, which can degrade the fluorescence intensity of fluorescent dye molecules.
Innovation Solution
A method is introduced that includes a display unit with separate regions for displaying live images and preview images, allowing users to predict the contents of the super-resolution image before construction, thereby optimizing imaging conditions and reducing the time required to obtain a desired super-resolution image.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple imaging attempts are performed to obtain a desired super-resolution image, then the probability of obtaining a satisfactory image increases, but the fluorescence intensity of fluorescent dye molecules attenuates due to repeated light irradiation
Solution Approach 1:
The system generates a preview image before constructing the final super-resolution image by extracting luminous point information from sequentially captured first images. This preliminary visualization allows users to determine whether the captured images are suitable for super-resolution construction, preventing unnecessary repeated imaging attempts that would further attenuate fluorescence intensity.
2Reliability
If users determine viewing field and imaging parameters through trial and error, then the ability to obtain desired super-resolution images improves, but the time required for imaging increases
Solution Approach 1:
The system provides real-time feedback by displaying a preview image generated from captured images before final super-resolution construction. Users can immediately see whether the captured images contain sufficient luminous points and whether the viewing field is appropriate, enabling informed decisions about parameter adjustment without waiting for final image construction or requiring multiple trial attempts.
3Manufacturing precision
If users repeatedly adjust imaging parameters and re-image, then the quality of super-resolution images improves, but the total imaging time increases
Solution Approach 1:
The preview image generation performs preliminary quality assessment of captured images by extracting and visualizing luminous point information. This allows users to determine image suitability before committing to time-consuming super-resolution construction, preventing wasted time on unsuitable images and reducing the need for repeated imaging cycles.
4Device complexity
If the system displays only the final super-resolution image, then the simplicity of the display interface is maintained, but the user's ability to predict and control image construction is reduced
Solution Approach 1:
The display interface is segmented into two distinct regions: a first display region showing preview images generated from captured data, and a second display region showing the final super-resolution image. This segmentation allows users to separately examine the preliminary captured images and the constructed super-resolution image, providing predictive capability and control while maintaining interface organization and clarity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables easier and more efficient acquisition of desired super-resolution images by allowing users to confirm and adjust imaging conditions before constructing the final image, thereby reducing the time and effort needed to achieve a suitable super-resolution image.
Implementation Method 1
in the case where the super-resolution image is constructed using images of fluorescence emitted from fluorescent dye molecules used to observe the sample
Data Source
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Figure 2A~2B
Figure 3
AI summary
A method according to one or more aspects may control a microscope system including an imaging unit configured to image a sample, and a display unit including a first display region and a second display region. The method may include: sequentially displaying, in the first display region, first images sequentially captured by the imaging unit; and displaying, in the second display region, a second image generated based on information extracted from the sequentially captured first images.