Microscope Temperature Monitoring for Focus Stability

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Existing tissue culture microscope apparatuses experience focus drift due to unmonitored temperature fluctuations of the microscope unit, leading to fuzzy images and positional shifts during cell observation.

Innovation Solution

Incorporating a microscope apparatus with a chamber, humidifier, chamber temperature sensor, microscope temperature sensor, and a determination device that assesses stability of chamber, humidifier, and microscope temperatures to determine if observation is possible, preventing focus drift by ensuring temperature stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Temperature

If temperature control is exerted only on the chamber without monitoring microscope temperature, then chamber temperature stability is improved, but microscope focus stability deteriorates due to unmonitored temperature fluctuations

Engineering Contradiction:
Improvechamber temperature stabilityVSAvoidfocus position stability
Core Design Contradiction:
TemperatureVSReliability

Solution Approach 1:

The patent implements temperature monitoring feedback by adding a microscope temperature sensor that continuously measures microscope unit temperature and feeds this information to a determination device. This feedback mechanism enables real-time detection of temperature fluctuations that cause focus drift, allowing the system to monitor and respond to temperature changes affecting focus stability.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The determination device acts as an intermediary between the temperature sensors and the observation system. It receives temperature data from both the chamber temperature sensor and microscope temperature sensor, processes this information to determine whether temperature conditions are suitable for observation, and provides guidance on when observation should be performed to ensure focus stability.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If multiple temperature sensors and monitoring systems are added to monitor both chamber and microscope temperatures, then focus stability is improved, but device complexity increases

Engineering Contradiction:
Improvefocus position stabilityVSAvoidtemperature monitoring system complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The determination device serves multiple functions: it receives data from both chamber and microscope temperature sensors, processes temperature information from multiple sources, determines suitability for observation, and provides guidance. This multi-functional approach consolidates what could be separate complex systems into a single integrated device, reducing overall system complexity while maintaining comprehensive temperature monitoring.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Prevents focus drift by maintaining stable temperatures within the microscope apparatus, ensuring clear and stable cell observations.

Implementation Method 1

a chamber temperature sensor that measures a temperature within the chamber

Methodology Applied
Scientific EffectTemperature measurement:

Implementation Method 2

a microscope temperature sensor that measures a temperature of the microscope unit

Methodology Applied
Scientific EffectTemperature measurement:

Implementation Method 3

a humidifier, connected to the chamber, that humidifies the interior of the chamber

Methodology Applied
Scientific EffectHumidification:

Data Source

PatentUS7628536B2Microscope apparatus
Publication Date: 2009.12.08 NIKON CORP
  • US7628536B2 patent drawing
  • US7628536B2 patent drawing
  • US7628536B2 patent drawing

AI summary

A microscope apparatus includes: a microscope unit; a chamber, arranged next to the microscope unit, that houses a specimen to be observed by the microscope unit; a humidifier, connected to the chamber, that humidifies the interior of the chamber; a chamber temperature sensor that measures a temperature within the chamber; a microscope temperature sensor that measures a temperature of the microscope unit; and a determination device that determines whether or not observation by microscope is possible based on outputs of the chamber temperature sensor and the microscope temperature sensor.