Microscope System Dynamic Resolution Matching
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Solution Overview
Problem
Current microscope systems face challenges in achieving optimal resolution and acquisition speed for 3D image data, as the numerical aperture of the microscope and the sampling pitch of the image-acquisition element are not dynamically controlled to match the acquisition purpose, leading to inefficiencies in data acquisition.
Innovation Solution
A microscope system that includes a variable-numerical-aperture microscope, an image-acquisition element, a purpose input unit, and a control unit that adjusts the numerical aperture and sampling pitch to ensure equal microscope and image-acquisition-element resolutions based on the acquisition purpose, allowing for high-speed or high-precision data acquisition by matching resolutions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the numerical aperture of the microscope is increased to improve resolution, then the microscope resolution value improves, but the acquisition speed decreases due to increased data volume
Solution Approach 1:
The system dynamically changes the numerical aperture parameter of the microscope based on the acquisition purpose. When high-speed acquisition is selected, the numerical aperture is reduced to decrease resolution and accelerate acquisition. When high-precision acquisition is selected, the numerical aperture is increased to improve resolution. This parameter adjustment resolves the contradiction between resolution and acquisition speed.
Solution Approach 2:
The system implements dynamic control of the numerical aperture and sampling pitch based on real-time acquisition purpose selection. The resolution control unit continuously adjusts these parameters to match the desired acquisition mode, making the system adaptable rather than static. This dynamic adjustment allows the system to optimize between resolution and speed based on operational requirements.
2Measurement precision
If the sampling pitch of the image-acquisition element is decreased to improve resolution, then the image-acquisition-element resolution value improves, but the acquisition speed decreases due to increased data processing requirements
Solution Approach 1:
The system dynamically adjusts the sampling pitch parameter of the image-acquisition element based on the acquisition purpose. When high-speed acquisition is selected, the sampling pitch is increased to reduce resolution and accelerate data acquisition. When high-precision acquisition is selected, the sampling pitch is decreased to improve resolution. This parameter adjustment resolves the contradiction between resolution and acquisition speed.
3Measurement precision
If both microscope and image-acquisition-element resolutions are optimized independently, then each component achieves its best performance, but the overall system efficiency decreases due to mismatched resolutions causing data bottlenecks
Solution Approach 1:
The resolution control unit implements a feedback mechanism that compares the microscope resolution value and image-acquisition-element resolution value, then adjusts the numerical aperture and sampling pitch to make them equal. This feedback loop ensures both components are coordinated optimally, preventing data bottlenecks and maximizing overall system efficiency while maintaining matched high performance.
Data Source
AI summary
A microscope system having a microscope forming an image of a specimen inserted onto an optical axis, an image-acquisition apparatus having an image-acquisition element which captures the image of the specimen, a purpose input unit with which an acquisition purpose of 3D image data is input, and a controller receiving the acquisition purpose, wherein the controller receives information about the numerical aperture of the microscope and information of a sampling pitch of the image-acquisition element, calculates a microscope resolution value and an image-acquisition-element resolution value on the basis of the received information, and sends at least one of the control signal for controlling the numeral aperture and the control signal for controlling the sampling pitch in response to the acquisition purpose to at least one of the microscope and the image-acquisition apparatus so that the calculated microscope resolution value and the calculated image-acquisition-element resolution value become the same.


