Scanning Microscope Pixel Sampling Synchronization
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Solution Overview
Problem
Existing scanning microscopes face challenges in sampling pixels in synchronization with the emission cycle of laser light, leading to brightness unevenness in generated images due to variations in the number of laser light emissions per pixel.
Innovation Solution
A scanning microscope that samples light from a sample irradiated with pulsed light and converts it into data for each pixel, where the sampling time per pixel is set to be an integer multiple of the pulse period of the pulsed light, and the sampling start timing is synchronized with a pixel synchronization signal.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the sampling time per pixel is set to synchronize with the pulse period of pulsed light, then the fluorescence signal including peak can be reliably sampled, but the sampling system becomes complex and difficult to adapt to different scanning conditions
Solution Approach 1:
The patent applies periodic action by setting the sampling time per pixel to be an integer multiple of the pulse period of the pulsed light. This ensures that sampling occurs at regular intervals that align with the periodic emission of laser pulses, allowing reliable capture of fluorescence signals including peaks while maintaining a simple, adaptable sampling system without complex synchronization mechanisms.
2Measurement precision
If the sampling start timing is synchronized with the pulse period of laser light, then the fluorescence signal can be reliably sampled, but brightness unevenness occurs in generated images due to variations in the number of laser light emissions per pixel
Solution Approach 1:
The patent resolves this contradiction by setting the sampling time per pixel to an integer multiple of the pulse period, creating a periodic sampling pattern that ensures a consistent number of laser pulse emissions are captured for each pixel. This periodic approach guarantees uniform brightness across the image while maintaining accurate fluorescence signal sampling, eliminating the brightness unevenness problem.
3Productivity
If the sampling time per pixel is shortened to achieve high-speed imaging, then imaging speed increases, but it becomes more difficult to synchronize with the laser emission cycle, causing brightness unevenness
Solution Approach 1:
The patent enables high-speed imaging while maintaining synchronization accuracy by setting the sampling time per pixel to an integer multiple of the pulse period. This periodic relationship ensures that even with short sampling times, each pixel consistently captures the same number of laser pulse emissions, preventing brightness unevenness and maintaining measurement precision despite the reduced sampling time required for high-speed imaging.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach eliminates or reduces brightness unevenness in the generated image, allowing for high-resolution and high-speed imaging without the need for synchronizing the sampling start timing with the pulse period of the laser light.
Implementation Method 1
a photodetector that detects light from the sample
Implementation Method 2
a laser light source that emits pulsed light
Data Source
AI summary
There is provided a scanning microscope that samples light from a sample irradiated with pulsed light and converts the light into data for each pixel, in which a sampling time per pixel is substantially an integer multiple of a pulse period of the pulsed light, and a sampling start timing of each pixel is synchronized with a pixel synchronization signal.