Optical Microscope Shading Correction via Reference Image
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Solution Overview
Problem
Optical microscopes present challenges in predicting and correcting lightness unevenness in output images due to their complex magnifying systems, making it difficult to apply existing shading correction techniques effectively.
Innovation Solution
An information processing apparatus that acquires lightness distribution information from a reference image of an observed area without a sample, using this data to correct lightness unevenness in images captured with the sample present, potentially storing this information for future use to shorten processing time.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If existing shading correction techniques using mathematical expressions are applied to optical microscope images, then correction processing can be performed, but the lightness unevenness caused by the complex magnifying optical system cannot be accurately predicted or corrected
Solution Approach 1:
The patent applies preliminary action by capturing a reference image of the observed area without a sample placed therein before actual sample observation. This reference image预先 records the lightness distribution characteristics of the optical system, which is then used to correct lightness unevenness in subsequent sample images through comparison and processing.
2Measurement precision
If lightness distribution information is acquired and stored for each observed area, then correction accuracy is improved, but processing time and storage requirements increase
Solution Approach 1:
The patent performs preliminary action by acquiring and storing lightness distribution information in advance for the observed area. This reference data is saved and reused for correcting multiple subsequent images, eliminating the need to recalculate correction parameters for each image and significantly reducing processing time while maintaining correction accuracy.
Data Source
AI summary
An information processing apparatus includes an acquisition section and a correction section. The acquisition section acquires information of lightness distribution of a first image captured by an imaging section capable of capturing an image of an observed area provided on an optical path of an optical system of an optical microscope, the image of the observed area being obtained by the optical microscope, the first image being an image of the observed area in a state where no sample is placed therein, the lightness distribution resulting from the optical system of the optical microscope. The correction section corrects, based on the information of lightness distribution acquired by the acquisition section, lightness unevenness of a second image captured by the imaging section, the second image being an image of the observed area in a state where the sample is placed therein.