Microscopic Object Segmentation Under Uneven Illumination

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Solution Overview

Problem

Existing micro-operation target segmentation methods suffer from low precision due to shadows caused by uneven illumination and image noise, compromising the accuracy of target recognition and positioning in micro-manipulation tasks.

Innovation Solution

A micro-operation-based target segmentation method using improved Otsu and edge operators, involving enhanced bilateral filtering and Otsu threshold segmentation to denoise and accurately separate foreground from background, while preserving edge information and correcting shadows.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional image processing techniques are used to enhance contrast and remove shadows, then the applicability is improved, but the computational time increases

Engineering Contradiction:
ImproveapplicabilityVSAvoidcomputational time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies bilateral filtering as a preliminary step before segmentation to pre-process the image and remove shadows caused by uneven illumination. This preliminary action simplifies subsequent segmentation operations by eliminating the need for complex shadow correction during the main processing stage, thereby reducing overall computational time while maintaining broad applicability across different imaging conditions

Inventive Principle:
Principle #10Preliminary action

2Measurement precision

If deep learning methods are used for shadow recognition and correction, then recognition accuracy is improved, but data support requirements increase

Engineering Contradiction:
Improverecognition accuracyVSAvoiddata support
Core Design Contradiction:
Measurement precisionVSQuantity of substance

Solution Approach 1:

The patent employs traditional image processing algorithms (bilateral filtering, Otsu thresholding, Canny edge detection) that require minimal computational resources and no large training datasets. These algorithms can be executed with simple, lightweight computational models rather than complex deep learning systems, achieving satisfactory shadow correction and segmentation accuracy without requiring extensive data support or powerful hardware resources

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

3Extent of automation

If threshold-based segmentation is used in micro-manipulation systems, then automation is achieved, but segmentation accuracy deteriorates due to shadows and noise

Engineering Contradiction:
Improveautomation levelVSAvoidsegmentation accuracy
Core Design Contradiction:
Extent of automationVSMeasurement precision

Solution Approach 1:

The patent divides the segmentation process into multiple distinct stages: first applying bilateral filtering to remove shadows and noise, then using Otsu thresholding for initial segmentation, followed by Canny edge detection to refine boundaries. This multi-stage segmentation approach maintains automation while significantly improving segmentation accuracy by addressing shadow and noise issues at each stage

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent introduces bilateral filtering as an intermediary processing step between image acquisition and threshold-based segmentation. This intermediary operation removes shadows and noise that would otherwise interfere with the thresholding process, enabling accurate automated segmentation even in the presence of uneven illumination

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS20260100018A1Micro-operation-based object segmentation method based on improved Otsu and edge operators
Publication Date: 2026.04.09 HARBIN UNIV OF SCI & TECH
  • US20260100018A1 patent drawing
  • US20260100018A1 patent drawing

AI summary

This invention presents an enhanced micro-operation target segmentation method utilizing improved Otsu and edge operators, addressing the limitations of conventional techniques in microscopic visual environments. The proposed approach effectively mitigates accuracy constraints caused by image noise and shadow effects from uneven illumination. The methodology comprises three key phases: Applying an optimized bilateral filtering algorithm to perform noise reduction on grayscale images, producing denoised images; Segmenting micro-operation targets using the refined Otsu algorithm on these denoised images to obtain initial segmentation results; Directly applying the final segmentation result when meeting predefined criteria, or conducting iterative segmentation through the enhanced edge operator and Otsu algorithm if necessary. This innovative method demonstrates exceptional performance in micro-operation target detection applications