Microscopy System Simultaneous Fluorescent Dye Concentration
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Solution Overview
Problem
Conventional microscopy systems require sequential detection of intensity distributions, which is time-consuming and prone to inaccuracies due to tissue changes during recording, especially in surgical contexts where the concentration of fluorescent dyes in tissues needs to be quickly and accurately determined.
Innovation Solution
A microscopy system that simultaneously records the intensity distributions of fluorescent light, reflected excitation light, and reflected emission light using distinct detection channels, allowing for the calculation of the actual intensity distribution of fluorescent light emitted by the dye, thereby determining the concentration of the dye in the tissue.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If sequential detection of intensity distributions is used, then device complexity is reduced, but measurement precision deteriorates due to tissue changes during recording
Solution Approach 1:
The detection system is segmented into multiple independent detection channels, each dedicated to detecting a specific intensity distribution (fluorescent light, reflected excitation light, reflected emission light) simultaneously. This segmentation allows parallel measurement without increasing overall system complexity, as each channel operates independently with its own detector and optical path.
Solution Approach 2:
The system transitions from sequential temporal detection to simultaneous spatial detection by adding multiple detection channels that operate in parallel. This dimensional change from time-based to space-based detection enables all intensity distributions to be recorded at the same moment, eliminating tissue changes during measurement while maintaining manageable device complexity through modular channel design.
2Productivity
If sequential detection is used, then device complexity is lower, but productivity deteriorates due to lengthy recording process
Solution Approach 1:
All detection channels operate continuously and simultaneously to detect their respective intensity distributions. This continuous parallel action eliminates the sequential waiting time between measurements, dramatically improving productivity by determining concentration in a single measurement cycle rather than through multiple sequential steps.
Solution Approach 2:
Multiple detection functions are merged into a single integrated system that processes all intensity distributions simultaneously. By combining the detection of fluorescent light, reflected excitation light, and reflected emission light into one coordinated system, the patent achieves rapid concentration determination without requiring separate sequential measurement procedures.
3Reliability
If sequential detection of intensity distributions is used, then device complexity is reduced, but reliability deteriorates due to tissue changes during recording
Solution Approach 1:
The detection system is segmented into multiple independent detection channels, each dedicated to detecting a specific intensity distribution (fluorescent light, reflected excitation light, reflected emission light) simultaneously. This segmentation allows parallel measurement without increasing overall system complexity, as each channel operates independently with its own detector and optical path.
Solution Approach 2:
The system transitions from sequential temporal detection to simultaneous spatial detection by adding multiple detection channels that operate in parallel. This dimensional change from time-based to space-based detection enables all intensity distributions to be recorded at the same moment, eliminating tissue changes during measurement while maintaining manageable device complexity through modular channel design.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate determination of the spatial distribution of fluorescent dye concentration, reducing the impact of tissue interference and improving the precision of concentration measurements in real-time surgical applications.
Implementation Method 1
a fluorescent dye is accumulated in the region of the diseased tissue, which, upon exposure to light of its excitation spectrum, emits fluorescent light of its emission spectrum
Implementation Method 2
the tissue absorbs light in the range of the excitation spectrum, and as a result not all the light provided for exciting the fluorescent dye actually reaches the fluorescent dye
Implementation Method 3
In the emission wavelength range, light scattering of the fluorescent light has the effect that not all the light that is emitted by the fluorescent dye reaches the detector, but is scattered by the surrounding tissue
Data Source
AI summary
A microscopy system has a detection system, which is configured to detect light of a first channel in a detection region and convert it to a first fluorescent light signal, to detect light of a second channel in a second detection region and convert it to a first correction signal, and to detect light of a third channel in a third detection region and convert it to a second correction signal. The system further includes a controller, which is configured to determine an approximation value for the spatial distribution of the concentration of the fluorescent dye in an object region using the first fluorescent light signal, the first correction signal, and the second correction signal. A first part of the emission spectrum of the fluorescent dye is detected in the first detection region.