Microspectroscope Position Correction via All-in-Focus Template Matching
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Solution Overview
Problem
Conventional microspectrometers face challenges in achieving accurate position correction, especially with unknown samples or foreign substances, due to positional deviations caused by component changes and long-time measurements, leading to inaccurate measurement results.
Innovation Solution
A microspectroscope that utilizes an all-in-focus image for position correction through template matching, incorporating a control unit with an image storage part to convert observation images into all-in-focus images and perform position corrections, and an objective lens system with a revolver for switching between low and high magnification lenses to address positional deviations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If position correction is performed using conventional methods with standard samples, then position correction information can be obtained, but the correction accuracy deteriorates for unknown samples or foreign substances due to focal point deviation in each region
Solution Approach 1:
The patent creates a digital copy of the standard sample image (template image) and uses template matching to find corresponding features in unknown sample images. This allows the system to transfer position correction information from the standard sample to unknown samples without requiring the unknown samples to be in focus at all times, resolving the contradiction between correction accuracy and adaptability to unknown samples
Solution Approach 2:
The system performs position correction based on pre-acquired standard sample images and stores the correction information in advance. When measuring unknown samples, the system retrieves and applies the pre-stored position correction information, eliminating the need for real-time focus adjustment and improving both accuracy and adaptability
2Productivity
If spectrometry is performed for a long duration, then more measurement data can be collected, but position deviation accumulates due to thermal expansion and component movement
Solution Approach 1:
The system acquires and stores position correction information in advance before long-time measurements begin. This preliminary action allows the system to compensate for position deviations caused by thermal expansion and component movement during extended measurements, maintaining precision while enabling high productivity
Solution Approach 2:
The system continuously monitors position deviations during measurements and uses the stored position correction information to adjust and correct positional drift in real-time, creating a feedback mechanism that maintains measurement precision throughout long-duration experiments
3Adaptability or versatility
If objective lens is switched between low and high magnification, then measurement flexibility is improved, but position deviation occurs due to mechanical movement
Solution Approach 1:
The system acquires position correction information when the objective lens is in one magnification state and stores it for use when switching to another magnification state. This preliminary acquisition of correction data before lens switching resolves the position deviation issue while maintaining measurement flexibility
Solution Approach 2:
The system creates and stores position correction information as a digital template that can be applied across different magnification states. When the objective lens switches between low and high magnification, the system retrieves the appropriate correction information, maintaining position accuracy despite the mechanical movement
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the accuracy of spectrometry by effectively correcting for position deviations, improving measurement precision and reducing errors associated with component changes and long-time measurements.
Implementation Method 1
a condensing lens that makes the excitation light incident on a predetermined position of the sample and condenses a reflected light or a transmitted light from the sample
Implementation Method 2
an objective lens that emits the excitation light to a predetermined position of the sample
Data Source
AI summary
The present invention relates to improvement in accuracy of an automatic sample detection technique in spectrometry of a microspectroscope.A microspectroscope 10 comprises: a light source 12 that emits an excitation light to a sample 20; a condensing lens 16 that emits the excitation light to a predetermined position of the sample 20 and condenses a reflected light or a transmitted light from the sample 20; a spectrometer 24 that detects a condensed light; and an analysis control unit 30 for analyzing a signal from the spectrometer 24; the microspectroscope 10 that uses an observation image of the sample 20 to perform spectrometry, whereinthe analysis control unit 30 comprises: an image storage part 32 that converts the observation image to an all-in-focus image to store the all-in-focus image; and a control part 34 that makes the microspectroscope 10 to perform measurement, andthe control part 34 uses the all-in-focus image and performs a template matching as a matching action of the image to perform position correction to a position deviation of a sample point that is a target of spectrometry in the sample.