Microtiter Plate Coating via Segmented Membrane Insertion

The microtiter plate is divided into modular components: a carrier layer with multiple wells, and separate membrane segments that can be independently prepared and then inserted into the wells. This segmentation allows the capture molecule coating process to be performed on flat membranes outside the narrow well spaces, avoiding the need for complex positioning tools within the wells while maintaining precise positioning through the modular assembly structure.

Multiple blot lines with different capture molecules are pre-applied to separate membrane segments outside the microtiter plate wells using standard line coating devices. These pre-prepared membrane segments are then inserted into the wells as complete units. This preliminary action eliminates the need for complex real-time positioning and dispensing operations within the narrow well spaces, significantly improving ease of operation while maintaining multi-parameter analysis capability through the diverse capture molecules on the membrane segments.

The production process is segmented into independent steps: preparing membrane segments with capture molecule coatings using standard devices, and then inserting these segments into microtiter plate wells. This segmentation enables parallel processing of multiple membrane segments, significantly improving productivity while maintaining flexibility in choosing the specific capture molecules to be applied on the membrane segments.

Instead of applying capture molecule coatings directly to the curved well bottoms where positioning is difficult and evaluation is problematic, the invention inverts the approach by applying the coatings to flat membrane segments outside the wells. The membrane segments are then inserted into the wells in a standardized orientation, ensuring both precise positioning and optimal conditions for digital image processing evaluation, as the blot lines are applied on flat surfaces with controlled geometry.