Microvesicle Isolation Using PEG Precipitation to Preserve Integrity

The patent replaces the mechanical ultracentrifugation system with a chemical precipitation system using polyethylene glycol (PEG). Instead of using high-speed rotation to separate microvesicles, the method uses PEG to induce precipitation of microvesicles from biological fluids through chemical interaction, thereby avoiding mechanical damage while achieving effective isolation

The patent changes the isolation parameter from mechanical force (ultracentrifugation speed and duration) to chemical concentration (PEG molecular weight and concentration). By adjusting PEG parameters such as molecular weight (e.g., 6000-8000) and concentration (e.g., 6-10% w/v), the method achieves microvesicle precipitation while maintaining their structural and functional integrity

The patent optimizes isolation parameters by using specific PEG molecular weights (6000-8000) and concentrations (6-10% w/v) to maximize microvesicle precipitation efficiency. This chemical parameter optimization enables obtaining sufficient quantities of microvesicles for both diagnostic and therapeutic applications while maintaining high isolation efficiency

The patent uses a composite approach by combining PEG precipitation with subsequent centrifugation or filtration steps. This multi-method composite strategy enhances both the quantity and purity of isolated microvesicles, ensuring sufficient material for various applications