Microvesicle Purification by Precipitation Without Integrity Loss
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Solution Overview
Problem
Existing methods for isolating microvesicles from biological fluids often damage their structural and functional integrity, leading to adverse reactions if used and are inefficient in providing sufficient quantities for diagnostic or therapeutic applications.
Innovation Solution
A method utilizing a precipitation agent to precipitate microvesicles from biological fluids by displacing water of solvation, followed by clarification, collection, and washing to maintain their integrity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If ultracentrifugation is used to isolate microvesicles, then microvesicles can be separated from biological fluids, but their structural and functional integrity is damaged
Solution Approach 1:
The invention changes the physical parameters of the isolation process by using precipitation agents (such as polyethylene glycol) to alter the solubility and phase state of microvesicles in the biological fluid, allowing them to precipitate out without the high-speed centrifugal forces that cause structural damage. This parameter change enables isolation while preserving integrity.
Solution Approach 2:
The invention replaces the mechanical ultracentrifugation system with a chemical precipitation system. Instead of using mechanical centrifugal force to separate microvesicles, the method uses chemical agents to induce precipitation, thereby eliminating the mechanical stress that damages microvesicle structure and function.
2Quantity of substance
If traditional isolation methods are used, then microvesicles can be obtained, but sufficient quantities for diagnostic or therapeutic applications are not provided
Solution Approach 1:
By adjusting parameters such as precipitation agent concentration, temperature, and pH, the invention optimizes the isolation process to maximize both the quantity of microvesicles obtained and the efficiency of the isolation procedure, providing sufficient quantities for diagnostic and therapeutic applications.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively isolates and purifies microvesicles without damage, enabling their use for therapeutic applications such as wound healing, tissue regeneration, and disease diagnosis, and enhances the preservation of their structural and functional integrity.
Implementation Method 1
A method utilizing a precipitation agent to precipitate microvesicles from biological fluids by displacing water of solvation
Data Source
AI summary
The present invention relates to the fields of medicine, cell biology, molecular biology and genetics. In particular, the present invention provides methods to isolate and purify microvesicles from cell culture supernatants and biological fluids. The present invention also provides pharmaceutical compositions of microvesicles to promote or enhance wound healing, stimulate tissue regeneration, remodel scarred tissue, modulate immune reactions, alter neoplastic cell growth and/or mobility, or alter normal cell growth and/or mobility. The present invention also provides compositions of microvesicles to be used as diagnostic reagents, and methods to prepare the compositions of microvesicles.