Microvolume Blood Dilution Solution for Stable GA Measurement
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Solution Overview
Problem
During transportation or storage of microvolume blood samples, the reaction between albumin and glucose can lead to changes in the glycated albumin (GA) value, affecting the accuracy of GA measurements.
Innovation Solution
A dilution solution containing a buffering agent and a salt is used to dilute the blood sample at a ratio greater than 8, inhibiting the glycation reaction and maintaining GA value accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If microvolume blood is transported or stored without dilution, then the sample volume is preserved, but the GA value changes due to glycation reaction between glucose and albumin
Solution Approach 1:
The patent changes the concentration parameter by diluting the blood sample with a buffering solution at a ratio of 9:1 or greater. This parameter change reduces the concentration of reactive glucose and albumin, thereby suppressing the glycation reaction and stabilizing the GA value during transportation and storage.
Solution Approach 2:
The patent introduces a buffering solution as an intermediary substance between the blood components (glucose and albumin). This intermediary dilutes the reactive components and creates a chemical environment that suppresses the unwanted glycation reaction, allowing stable transport of the blood sample.
2Stability of the object's composition
If the blood sample is diluted to prevent glycation, then the GA value stability is improved, but the blood concentration decreases
Solution Approach 1:
The patent optimizes the dilution ratio parameter to 9:1 or greater, which is sufficient to suppress glycation reactions while preserving adequate blood component concentrations for accurate GA measurement. This parameter optimization resolves the contradiction between stability and concentration.
3Measurement precision
If a large amount of blood is collected for testing, then the measurement accuracy is improved, but the invasiveness increases
Solution Approach 1:
The patent performs dilution with buffering solution as a preliminary action immediately after blood collection. This preliminary dilution stabilizes the sample and prevents glycation, allowing accurate GA measurement from smaller blood volumes, thereby reducing invasiveness while maintaining measurement precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively stabilizes the GA value during transportation and storage, allowing accurate measurement with minimal sample loss and reduced invasiveness, suitable for HPLC methods.
Implementation Method 1
the solution may contain a buffering agent and a salt as active ingredients
Implementation Method 2
the solution may be configured to dilute the blood at a dilution ratio of greater than 8
Data Source
AI summary
According to an embodiment of the present disclosure, a solution for transporting or storing a blood sample is provided. The blood sample may be a microvolume blood sample. In some embodiments, the solution may contain a buffering agent and a salt as active ingredients. The solution may be configured to dilute the blood at a dilution ratio of 10 or greater.