Microwell Filling Speed Control for Uniform Capture Substance Deposition
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Solution Overview
Problem
Existing detection methods for target substances in microwells suffer from low filling efficiency, uneven distribution, and reduced detection sensitivity due to aggregation and deposition of target-capturing substances outside the microwells, which inhibits the flow of the dispersion medium.
Innovation Solution
A method and system that control the introduction speed of a dispersion medium containing the target-capturing substance based on capturing substance information, using a system to determine the introduction speed, ensuring efficient filling of microwells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If the introduction speed of the dispersion medium is not controlled, then the filling process is simple, but the target-capturing substance aggregates and deposits outside the microwells, reducing detection sensitivity
Solution Approach 1:
The patent controls the introduction speed of the dispersion medium as a critical parameter to prevent aggregation and deposition of the target-capturing substance. By optimizing the flow rate parameters during the filling process, the system achieves uniform distribution of particles in the microwells without requiring complex additional devices.
2Productivity
If the introduction speed is increased to improve filling efficiency, then the filling time is reduced, but the target-capturing substance aggregates outside the microwells
Solution Approach 1:
The patent identifies the introduction speed as a critical parameter that balances filling efficiency and uniformity. By optimizing this parameter within a specific range, the system achieves both high productivity and uniform distribution, preventing aggregation while maintaining efficient filling.
Solution Approach 2:
The system dynamically adjusts the introduction speed of the dispersion medium based on the specific characteristics of the target-capturing substance. This dynamic control allows the system to adapt to different substances and conditions, maintaining optimal filling efficiency and uniformity for each case.
3Manufacturing precision
If the introduction speed is decreased to improve filling uniformity, then the filling precision is improved, but the filling time increases
Solution Approach 1:
The patent optimizes the introduction speed parameter to achieve the best balance between filling uniformity and time consumption. Rather than using excessively low speeds, the system identifies an optimal speed range that provides sufficient uniformity while maintaining reasonable filling time.
4Measurement precision
If no speed control is implemented, then the system is simple to operate, but detection sensitivity is reduced due to aggregation and deposition
Solution Approach 1:
The patent controls the introduction speed as a key parameter to prevent aggregation and deposition, thereby maintaining high detection sensitivity. This parameter control is implemented through straightforward means that do not significantly complicate the operation of the system.
Data Source
AI summary
Provided is a filling method for filling a microwell with a target-capturing substance, the filling method including: a capturing substance information acquisition step of acquiring capturing substance information relating to a target-capturing substance to be used; a speed determination step of determining, based on the capturing substance information acquired in the capturing substance information acquisition step, an introduction speed at which a dispersion medium in which the target-capturing substance is dispersed is introduced to a region on a base material in which a plurality of the microwells are formed, the region including the plurality of the microwells formed therein; and a capturing substance filling step of filling one or more of the plurality of the microwells with the target-capturing substance by introducing the dispersion medium to the base material at the introduction speed.