Mid-Infrared Cell Sample Evaluation via Photothermal Dead Cell Counting
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Solution Overview
Problem
Current techniques lack an effective method for evaluating the culture state of cell samples, particularly due to cell damage and stress during passage operations, which affects the quality and differentiation capacity of cultured cells, making it difficult to assess the culture state accurately.
Innovation Solution
A cell sample evaluation method and apparatus utilizing mid-infrared light irradiation to measure dead cell numbers, correlating irradiation amount with culture state, and comparing these correlations between a reference and object cell sample to evaluate the culture state effectively.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If passage operation is performed to transfer cultured cells to new medium, then cell proliferation can continue, but cell quality and state deteriorate due to damage and stress
Solution Approach 1:
The patent applies preliminary action by performing light irradiation treatment on cells before passage operation to reduce stress and damage during transfer. The cells are pre-adapted to light exposure, which enhances their tolerance to the environmental changes occurring during passage, thereby maintaining cell quality while enabling continued proliferation.
Solution Approach 2:
The patent utilizes parameter changes by varying light irradiation conditions (wavelength, intensity, duration) to optimize cell state for passage. By adjusting these physical parameters, the cells are prepared in a more resilient state, reducing the negative impact of passage operations on cell quality while maintaining productivity.
2Productivity
If passage number increases to maintain cell supply, then cell quality deteriorates due to changes in differentiation and proliferation capacity, but cell availability is maintained
Solution Approach 1:
The patent replaces traditional mechanical and chemical cell quality assessment methods with optical measurement using light absorption spectroscopy. This non-invasive optical system continuously monitors cell state parameters without disturbing the cells, enabling accurate quality assessment even at high passage numbers while maintaining cell availability.
Solution Approach 2:
The patent introduces light absorption characteristics as an intermediary parameter to assess cell quality. Instead of directly measuring complex cellular properties, the system uses light absorption at specific wavelengths as a mediator that reflects cell state, providing a reliable indirect measurement method that works effectively across multiple passage numbers.
3Loss of information
If traditional cell evaluation methods are used, then cell quality assessment is difficult due to lack of established techniques, but existing methods do not provide accurate culture state information
Solution Approach 1:
The patent replaces complex mechanical and chemical evaluation systems with a simplified optical measurement system. By using light absorption spectroscopy, the system obtains comprehensive culture state information through non-invasive optical measurements, reducing device complexity while preventing information loss about cell culture conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables suitable evaluation of the culture state of cell samples by measuring dead cell numbers generated from mid-infrared light irradiation, providing an index for assessing passage number and condition, thereby improving the accuracy of cell sample evaluation.
Implementation Method 1
irradiating an irradiation region set in a culture region of a plurality of cells contained in an object cell sample with evaluation light having a wavelength in a mid-infrared region
Implementation Method 2
measuring a dead cell number generated according to an irradiation amount of the evaluation light
Data Source
AI summary
A cell sample evaluation apparatus includes an evaluation light irradiation unit for irradiating an irradiation region set in a culture region of a plurality of cells in an object cell sample with evaluation light having a wavelength in a mid-infrared region under a predetermined irradiation condition, a dead cell number measurement unit for measuring a dead cell number generated according to an irradiation amount of the evaluation light in the irradiation region of the object cell sample, and a culture state analysis unit for evaluating a culture state of the object cell sample by obtaining an object correlation between the irradiation amount of the evaluation light and the dead cell number for the object cell sample and comparing a reference correlation between the irradiation amount of the evaluation light and the dead cell number for a reference cell sample and the object correlation.