Mie Light Scattering Microorganism Detection
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Solution Overview
Problem
Conventional methods for detecting foodborne pathogens are time-consuming due to requirements for sample preparation and concentration, such as cell lysis and filtration, and often involve complex processes that are not efficient for rapid detection.
Innovation Solution
A method and device utilizing antibody-conjugated beads and Mie forward light scattering to detect microorganisms, where beads with specific antibodies are mixed with a sample, irradiated with light, and the scattered light is measured at specific angles to determine the presence of microorganisms, allowing for rapid and efficient detection without extensive sample preparation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional detection methods are used (cell lysis, filtration, and concentration), then detection reliability is improved, but detection time increases and productivity decreases
Solution Approach 1:
The invention extracts and eliminates the time-consuming sample preparation steps (cell lysis, filtration, concentration) from the detection workflow. By using whole sample analysis with antibody-conjugated beads that directly bind to microorganisms in the original sample, the method achieves rapid detection without sacrificing reliability, as the immunological detection mechanism remains intact while removing unnecessary preprocessing steps.
Solution Approach 2:
The invention applies preliminary action by pre-conjugating antibodies to beads before the detection process. This pre-prepared reagent system allows for direct addition to the sample, eliminating the need for sample preparation steps. The antibody-beads are ready to immediately bind and detect microorganisms upon contact with the sample, achieving both speed and reliability.
2Measurement precision
If conventional detection methods are used (cell lysis, filtration, and concentration), then detection accuracy is improved, but process complexity increases
Solution Approach 1:
The invention removes the complex sample preparation apparatus and procedures (lysis equipment, filtration systems, concentration devices) from the detection process. By using a simple mixing approach where antibody-conjugated beads directly interact with microorganisms in the original sample, the method maintains detection accuracy through specific immunological binding while dramatically reducing process complexity to basic mixing and reading steps.
Solution Approach 2:
The invention replaces complex mechanical sample preparation systems with a biochemical detection system. Instead of using mechanical lysis, filtration, and concentration steps, the method uses antibody-conjugated beads that chemically and specifically bind to microorganisms through immunological recognition, achieving accurate detection through molecular interaction rather than mechanical processing.
3Reliability
If extensive sample preparation is performed (cell lysis, filtration, and concentration), then detection reliability is improved, but productivity decreases
Solution Approach 1:
The invention extracts and eliminates the time-consuming sample preparation steps (cell lysis, filtration, concentration) from the detection workflow. By using whole sample analysis with antibody-conjugated beads that directly bind to microorganisms in the original sample, the method achieves rapid detection without sacrificing reliability, as the immunological detection mechanism remains intact while removing unnecessary preprocessing steps.
Solution Approach 2:
The invention enables continuous detection by eliminating the batch-wise sample preparation steps. The antibody-conjugated beads can be directly added to samples in a continuous flow or sequential manner, allowing multiple samples to be processed back-to-back without interruption for preparation steps, thereby significantly increasing detection throughput and productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and efficient detection of microorganisms in food samples by measuring changes in light scattering, reducing the need for extensive sample preparation and providing a portable and cost-effective solution for identifying foodborne pathogens.
Implementation Method 1
The device may quantify increased light scattering due to immunoagglutination in the device (e.g., immunoagglutination in a sample in the device). detecting a forward scattered light scattered by the first mixture, the forward scattered light is at a first angle with respect to the first incident light, the first angle being between about 30 to 60 degrees
Data Source
AI summary
The present invention features methods and devices for microorganisms through detecting Mie light scattering from immunoagglutinated beads. The methods feature providing a first bead suspension with antibody specific for the microorganism conjugated to beads; mixing the first bead suspension with a sample to form a first mixture; irradiating the first mixture with first incident light; detecting forward light scattering at a first angle with respect to the first incident light, where the first angle being between about 30 to 60 degrees; determining I from the light scattering; providing a second bead suspension with no antibody and simultaneously measuring I0 in a similar manner; comparing I with I0. All light scattering measurements may be made in a two-well slide or a Y-channel microfluidic device.