Pearl Millet 53-1-1 Purple Plant 3 Gene Pigmentation

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current pearl millet cultivars lack a consistent and intense purple pigmentation that is not initially photo-dependent, limiting their aesthetic appeal and potential uses in ornamental and nutritional applications.

Innovation Solution

Development of the pearl millet cultivar 53-1-1, which exhibits intense purple coloration in leaves, stems, and grains due to the novel Purple Plant 3 (PP3) gene, where pigment formation initiates before emergence and is intensified by light, differing from existing cultivars.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If conventional pearl millet cultivars are used, then the plant can complete its growth cycle and produce grain, but the purple pigmentation is either absent or weak and not stable

Engineering Contradiction:
Improvepurple pigmentation stabilityVSAvoidpigmentation consistency
Core Design Contradiction:
Ease of manufactureVSReliability

Solution Approach 1:

The patent introduces a novel gene (PP3) that changes the genetic parameters of the plant, resulting in stable and intense purple pigmentation throughout the plant tissues. This gene modification transforms the conventional cultivar characteristics to achieve consistent coloration that maintains stability across different growth stages and environmental conditions.

Inventive Principle:
Principle #35Parameter changes

2Ease of manufacture

If the purple pigmentation is intensified, then the aesthetic appeal and nutritional value are enhanced, but the breeding process becomes more complex

Engineering Contradiction:
Improvepigmentation intensityVSAvoidbreeding process complexity
Core Design Contradiction:
Ease of manufactureVSDevice complexity

Solution Approach 1:

The patent extracts and isolates the specific gene responsible for purple pigmentation (PP3) from the complex genetic background. By identifying and separating this single gene, the breeding process is simplified, as farmers can focus on introducing this specific gene rather than managing complex multi-gene inheritance patterns.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses a carrier line (53-1-1) that acts as an intermediary to transfer the PP3 gene to other cereal varieties. This carrier line simplifies the breeding process by providing a stable platform that can be crossed with different varieties to introduce the purple pigmentation trait without requiring complex direct breeding programs.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Ease of manufacture

If pigment formation initiates before emergence, then the coloration is more intense and widespread, but the photo-dependency is reduced

Engineering Contradiction:
Improvecoloration intensityVSAvoidphoto-dependency reduction
Core Design Contradiction:
Ease of manufactureVSObject-affected harmful factors

Solution Approach 1:

The patent implements preliminary action by initiating pigment formation before the plant emerges from the soil. The PP3 gene activates pigment synthesis during the embryonic stage and early root development, ensuring that purple coloration is already established in roots, stems, and leaves before emergence. This preliminary pigment accumulation creates intense and widespread coloration that is not dependent on post-emergence light exposure.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The PP3 gene in cultivar 53-1-1 provides a stable, dominant purple coloration that is more intense and widespread within the plant, enhancing horticultural appeal and nutritional value, and can be used in breeding to introduce purple pigmentation into other cereal varieties.

Implementation Method 1

The 53-1-1 line also has a novel gene, designated Purple Plant 3 (PP3), which results in the purple pigmentation that develops in the leaf, stem, head and grain.

Methodology Applied
Scientific EffectAnthocyanin pigment formation:

Implementation Method 2

the initial formation of pigment is not light induced, subsequent exposure to light further increases production to higher levels of intensity not found in other purple pearl millets

Methodology Applied
Scientific EffectPhoto-dependent pigment intensification: Photosynthesis

Data Source

PatentUS7750214B2Pearl millet line 53-1-1 with PP3 gene and all derivatives produced by any method
Publication Date: 2010.07.06 BALL HORTICULTURAL CO
  • US7750214B2 patent drawing
  • US7750214B2 patent drawing
  • US7750214B2 patent drawing

AI summary

The invention relates to a pearl millet plant, seed, variety, parental line and hybrid. More specifically the invention relates to a pearl millet plant having a mutant allele that increases purple pigmentation in many plant parts. The invention relates to crossing inbreds, varieties, and hybrids containing the purple allele to produce novel types and hybrids of pearl millet and other compatible millet species, both for pigment extraction and ornamental purposes.