miRNA Plasmid Composition for CXCR4 Expression Control
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Solution Overview
Problem
Bioactive molecules, such as CXCR4, when over-expressed or mis-expressed, disrupt homeostasis leading to disease, necessitating therapies to regulate their expression.
Innovation Solution
Compositions comprising recombinant plasmids that encode miRNA sequences, delivered via vectors like AAV, to upregulate miRNA production, targeting and degrading CXCR4 mRNA, thereby reducing CXCR4 expression.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If CXCR4 is over-expressed to enhance immune response, then immune response is improved, but homeostasis is lost leading to disease
Solution Approach 1:
The patent employs miRNA as a negative feedback mechanism to regulate CXCR4 expression. When CXCR4 expression becomes excessive, the introduced miRNA sequences bind to CXCR4 mRNA and degrade it, reducing CXCR4 protein levels back to homeostatic ranges. This feedback loop prevents both over-expression and under-expression, maintaining optimal immune function while avoiding disease states.
Solution Approach 2:
The patent changes the expression parameter of CXCR4 by introducing miRNA sequences that specifically target CXCR4 mRNA. This parameter change allows dynamic regulation of CXCR4 levels - increasing them when immune response is needed and decreasing them when homeostasis requires reduction, thereby resolving the contradiction between enhanced immune response and maintenance of homeostasis.
2Productivity
If miRNA production is upregulated to suppress target biomolecule, then target biomolecule expression is reduced, but production complexity increases
Solution Approach 1:
The patent uses self-service by leveraging the cell's own transcriptional and translational machinery to produce miRNA. The introduced DNA sequences containing miRNA coding regions are transcribed by cellular RNA polymerase and processed by cellular Dicer enzymes into functional miRNA. This self-service approach avoids the need for complex external production systems while achieving high miRNA production levels.
Solution Approach 2:
The patent introduces DNA sequences as an intermediary carrier that contains the miRNA coding regions. These DNA intermediaries are introduced into cells and serve as templates for miRNA production. This intermediary approach simplifies the overall system by using a stable, easy-to-deliver DNA molecule rather than directly introducing complex miRNA production machinery.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The compositions effectively downregulate CXCR4 expression, restoring homeostasis and potentially treating diseases associated with dysregulated CXCR4 activity.
Implementation Method 1
The sequences of miRNA may be complimentary to a sequence of target messenger RNA (mRNA) that encodes for translation of a target biomolecule and the miRNA can cause the target mRNA to be degraded or inactivated
Data Source
AI summary
The embodiments of the present disclosure relate to one or more compositions or methods that upregulate the production of one or more sequences of micro-interfering ribonucleic acid (miRNA). The sequences of miRNA may be complimentary to a sequence of target messenger RNA (mRNA) that encodes for translation of a target biomolecule, such as CXCR4. The miRNA can cause the target mRNA to be degraded or inactivated, thereby causing a decrease in bioavailability of the target biomolecule because it is degraded or inactivated by the miRNA. Decreasing the bioavailability of the target biomolecule within a subject that is administered the one or more compositions may address the afflictions experienced by the subject due to expression of the target biomolecule.