Mirror-Based Fluorescence Reading for Chromatic Aberration Control
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Solution Overview
Problem
Existing fluorescence reading devices face challenges in efficiently detecting and correcting chromatic aberrations, particularly when observing multi-wavelength fluorescence from microarrays, which leads to image blurring and increased costs due to the need for complex lens systems.
Innovation Solution
The fluorescence reading device employs a receiving optical system composed of a convex mirror and a concave mirror, eliminating the need for lenses and reducing chromatic aberration effects. This system allows for precise image formation on the CCD camera without the complexity and cost associated with lens-based systems.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a lens system is used for fluorescence detection, then image formation capability is improved, but chromatic aberration increases and manufacturing cost increases
Solution Approach 1:
The patent removes the lens component from the optical system entirely, extracting the source of chromatic aberration while maintaining image formation capability through a mirror-based relay optical system. This eliminates the harmful chromatic effects inherent in lens-based systems.
Solution Approach 2:
The patent replaces the lens-based optical system with a mirror-based relay optical system. By substituting refractive optics (lenses) with reflective optics (mirrors), the system achieves chromatic aberration-free image formation while maintaining the necessary optical functionality.
2Measurement precision
If a lens system is used for fluorescence detection, then image formation capability is improved, but device complexity and manufacturing cost increase
Solution Approach 1:
The patent extracts and removes the lens component from the optical system, simplifying the overall device structure. By eliminating the lens, the system reduces manufacturing complexity and cost while maintaining image formation through the relay optical system using mirrors.
3Adaptability or versatility
If multi-wavelength fluorescence detection is performed, then detection versatility is improved, but chromatic aberration and image blurring increase
Solution Approach 1:
The patent replaces the lens-based system with a mirror-based relay optical system, which is achromatic and does not suffer from chromatic aberration. This enables high-quality multi-wavelength fluorescence detection without the image blurring that plagues lens-based systems when detecting multiple wavelengths.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The proposed optical system effectively reduces chromatic aberration, enhances sensitivity in detecting fluorescence, and lowers production costs by simplifying the optical design, thereby improving the accuracy and efficiency of fluorescence reading.
Implementation Method 1
a receiving optical system comprising a convex mirror and a concave mirror
Data Source
AI summary
There is provided a fluorescence reading device which acquires fluorescence from a microarray, including: a receiving optical system which repeatedly reflects fluorescence emitted from the microarray off a plurality of reflectors to form an image on an image-forming plane; and an optical sensor located on the image-forming plane. The fluorescence reading device may be a telecentric optical system in which an image-capturing plane of the microarray and an image-forming plane are the same in size. The plurality of reflectors may include a convex mirror and a concave mirror. The plurality of reflectors may include only one convex mirror and one concave mirror.