Mirror Image Microscopic Imaging Device for Microneedle Calibration
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Solution Overview
Problem
Conventional ICSI methods rely on manual microneedle posture adjustment, which is subjective and difficult to achieve the ideal posture due to limitations in observing the microneedle's position in horizontal view, leading to potential damage to oocytes and poor manipulation results.
Innovation Solution
A microscopic mirror imaging device with a motion actuator and mirror image former, integrated with an inverted microscope system and micromanipulation system, allows for automatic calibration of the microneedle posture by forming a mirror image and analyzing angles in both plan and horizontal views, enabling precise adjustment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If manual microneedle posture adjustment is used, then the operation is simple, but the measurement precision and manufacturing precision of microneedle posture are poor
Solution Approach 1:
A mirror image former is introduced as an intermediary component to enable indirect observation of the microneedle's lower edge in horizontal view. The mirror reflects light from the lower edge to the eyepiece, allowing operators to see the horizontal plane alignment that would otherwise be invisible, thereby improving measurement precision without complicating the operation
Solution Approach 2:
The invention adds a new observation dimension by incorporating a mirror at a 45-degree angle to the optical axis. This allows the lower edge of the microneedle, which is normally invisible in the standard vertical optical path, to be observed in horizontal view, enabling precise posture measurement in an additional dimensional space
2Device complexity
If manual microneedle posture adjustment is used, then the device complexity is low, but the manufacturing precision of microneedle posture is poor
Solution Approach 1:
The mirror image former acts as a simple intermediary that enables precise posture alignment by making the lower edge visible. This single component addition allows operators to achieve accurate horizontal plane alignment without requiring complex automated positioning systems, maintaining low device complexity while improving manufacturing precision
3Measurement precision
If automated mirror image calibration is implemented, then the measurement precision and manufacturing precision are improved, but the device complexity increases
Solution Approach 1:
The mirror image former serves as a straightforward intermediary component that enables precise measurement of the microneedle's lower edge position and angle. By reflecting light from the lower edge to the eyepiece, it provides direct visual feedback for calibration without requiring complex sensors, cameras, or computational systems
Solution Approach 2:
The system enables self-calibration through direct visual observation. The operator can see the mirror image of the lower edge in the eyepiece and manually adjust the microneedle posture to achieve the desired horizontal alignment, eliminating the need for complex automated calibration systems with multiple sensors and control algorithms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables precise calibration of the microneedle's posture, ensuring the lower edge is parallel to the horizontal plane, reducing damage to oocytes and improving micromanipulation results through automated and objective adjustment.
Implementation Method 1
a plane mirror (1911) mounted on the other end of the mirror image former support (192). An angle formed between a mirror surface of the plane mirror (1911) and a horizontal plane of the microscope stage is equal to 45°
Data Source
AI summary
There are provided a microscopic mirror imaging device, a system and a method for calibrating a posture of a microneedle. The microscopic mirror imaging device includes a motion actuator, a mirror image former support and a mirror image former. The motion actuator is fixedly mounted on a microscope stage. One end of the mirror image former support is connected to the motion actuator. The mirror image former includes a plane mirror mounted on the other end of the mirror image former support. An angle formed between a mirror surface of the plane mirror and a horizontal plane of the microscope stage is equal to 45°, and an angle formed between the mirror surface of the plane mirror and a coronal plane of the microscope stage is equal to 45°.