Mitochondria-Coated Culture Surfaces for Efficient Cell Uptake
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Solution Overview
Problem
Existing methods for introducing exogenous mitochondria into cells face challenges such as low efficiency, cell damage, and require advanced techniques, limiting their applicability and effectiveness.
Innovation Solution
A production method involving culturing recipient cells using cell culture equipment with a culture surface coated with isolated mitochondria, utilizing centrifugation to attach mitochondria, and optimizing conditions for efficient incorporation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If exogenous mitochondria are incubated with recipient cells, then the operation is easy, but introduction efficiency is low
Solution Approach 1:
The patent uses cell culture equipment as an intermediary carrier to facilitate mitochondrial introduction. The equipment surface is treated to enable mitochondrial attachment, and recipient cells are cultured on this surface, allowing mitochondria to be introduced during the culturing process without direct manipulation
Solution Approach 2:
The patent applies preliminary treatment to the cell culture equipment surface before introducing mitochondria. The surface is coated or treated with specific materials to create favorable conditions for mitochondrial attachment and subsequent cell incorporation, preparing the environment in advance for efficient mitochondrial introduction
2Productivity
If the surface of exogenous mitochondria is modified with cell-penetrating peptide, then introduction efficiency is higher, but cells are damaged due to cationic substance
Solution Approach 1:
The patent extracts the cell-penetrating peptide modification step from the mitochondrial introduction process. Instead of modifying mitochondria with cationic peptides, the method uses natural mitochondrial properties combined with treated culture surfaces to achieve introduction without harmful chemical modifications
Solution Approach 2:
The patent converts the negative charge of mitochondrial surfaces, which normally hinders introduction, into a beneficial feature by using it for controlled attachment to positively treated culture surfaces, transforming a barrier into a mechanism for efficient and non-damaging introduction
3Reliability
If exogenous mitochondria are introduced by microinjection, then mitochondria can be certainly introduced, but advanced technique is required and number of recipient cells is limited
Solution Approach 1:
The patent enables recipient cells to self-incorporate mitochondria during the culturing process on treated surfaces. The cells perform the introduction function themselves through natural processes during culture, eliminating the need for complex microinjection techniques and allowing large numbers of cells to be processed simultaneously
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for high-efficiency introduction of exogenous mitochondria into cells with minimal damage, enhancing cell function and viability, suitable for cell medicine and regenerative medicine applications.
Implementation Method 1
cell culture equipment in which the culture surface is coated with mitochondria isolated from donor cells
Data Source
AI summary
A method of producing cells into which exogenous mitochondria have been introduced, the production method including culturing recipient cells using cell culture equipment in which mitochondria isolated from donor cells are coated on the culture surface, and incorporating the mitochondria into the recipient cells. Cells produced by the method, the cells having exogenous mitochondria introduced thereinto; a cell culture substrate usable in the aforesaid production method and a method for manufacturing the same; and a cell culture kit.